The reliability and stability of array slides are a big concern for array vendors and end users. Herein, we report on a new type of array slide with high reactivity toward DNA probes and low side reaction. A one-step surface reaction via the Michael addition involved in preparing array slides was developed and characterized by x-ray photoelectron spectroscopy, contact angle, and fluorescence labeling. The effects of array fabrication and storage conditions, i.e., spotting solution pH, high humidity, and long-term storage on the reactivity of the slides were examined. The fabricated DNA arrays could realize good hybridization efficiency (38.2% for slides with 0.88 pmol/cm2), low limit of detection (4 × 10-14M), as well as high mismatch selectivity.

中文翻译：

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通过表面迈克尔加成法制备和表征DNA阵列玻片。

阵列载玻片的可靠性和稳定性是阵列供应商和最终用户的主要关注点。在本文中，我们报道了一种新型的阵列载玻片，其对DNA探针的反应性高且副反应低。开发了通过迈克尔加成反应的一步式表面反应，该反应涉及制备阵列载玻片，并通过X射线光电子能谱，接触角和荧光标记进行了表征。检查了阵列制造和储存条件（即点样溶液的pH值，高湿度和长期储存）对载玻片反应性的影响。制备的DNA阵列可以实现良好的杂交效率（对于0.88 pmol / cm2的载玻片为38.2％），低检测限（4×10-14M）以及高错配选择性。