Engineered scaffolds simultaneously exhibiting multiple cues are highly desirable for neural tissue regeneration. Silk fibroin is a promising natural protein material for nerve repair. However, the lack of specific bioactive cues significantly hinders its application. In this study, the electrospun silk fibroin nanofibers with both biochemical and topographical cues were prepared. The alignment of electrospun nanofibers was optimized by controlling the surface linear velocity of a rotating drum. The silk fibroin nanofibers were further functionalized with laminin through covalent binding, confirmed by immunostaining observation. Cell proliferation and neurite outgrowth assays confirmed that the functionalized aligned nanofibers significantly enhanced directional axonal extensions, providing physical and bioactive cues for neurite outgrowth. Furthermore, the tubular scaffolds with longitudinally aligned microchannels were designed by rolling the functionalized silk fibroin nanofibers. The neurite extension across the lumen of the conduit along the direction of the aligned fibers is apparent. These results highlight the ability of laminin-immobilized silk fibroin nanofibers to enhance neurite outgrowth and to control directional neurite extension, providing a useful approach to construct a regenerative microenvironment for nerve repair materials.

中文翻译：

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生物功能化丝素蛋白纳米纤维，用于定向生长和长轴突生长。

同时展现多种线索的工程支架对于神经组织再生是非常需要的。丝素蛋白是用于神经修复的有前途的天然蛋白质材料。但是，缺乏特定的生物活性提示明显阻碍了其应用。在这项研究中，准备了具有生化和地形学提示的静电纺丝素蛋白纳米纤维。通过控制转鼓的表面线速度，优化了电纺纳米纤维的排列。丝素蛋白纳米纤维通过层粘连蛋白通过共价结合进一步功能化，通过免疫染色观察证实。细胞增殖和神经突生长试验证实，功能化的对齐纳米纤维显着增强了轴突的定向延伸，为神经突生长提供了物理和生物活性线索。此外，通过滚动功能化的丝素蛋白纳米纤维来设计具有纵向排列的微通道的管状支架。沿排列的纤维方向在导管内腔上的神经突延伸是明显的。这些结果突出了层粘连蛋白固定的丝素蛋白纳米纤维增强神经突向外生长并控制神经突定向延伸的能力，为构建神经修复材料的再生微环境提供了有用的方法。