Parkinson's disease (PD) is a neurodegenerative disorder characterized by the accumulation of α-synuclein into Lewy bodies. 3-Benzylidine-indolin-2-one represent a class of compounds, which are known to inhibit the accumulation of α-synuclein. In this paper we report the synthesis of [13 C] and [15 N] labelled ​1-benzyl​-​(Z)-3-​​(benzylidene)indolin-2-one from commercially available [13 C2 ]-chloroacetic acid and [15 N]-aniline in five steps. The product will be used to study its metabolites in human liver microsomes by liquid chromatography-tandem mass spectrometry.

中文翻译：

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[13 C2, 15 N]-1,3- 2H - 1-benzyl - (Z )-3-( benzylidene)indolin-2-one的合成

帕金森病 (PD) 是一种神经退行性疾病，其特征在于 α-突触核蛋白在路易小体中的积累。3-Benzylidine-indolin-2-one 代表一类化合物，已知它们可以抑制 α-突触核蛋白的积累。在本文中，我们报道了从市售的 [13 C2 ]-氯乙酸中合成 [13 C] 和 [15 N] 标记的 1-苄基 - (Z)-3- (亚苄基)indolin-2-one酸和 [15 N]-苯胺分五个步骤。该产品将用于通过液相色谱-串联质谱法研究其在人肝微粒体中的代谢物。