Much evidence demonstrates the antinociceptive effect of magnetic fields (MFs). However, the analgesic action mechanism of the electromagnetic field (EMF) is not exactly understood. The aim of the present study was to investigate the effects of 5-HT1 and 5-HT2 receptor agonists (serotonin HCl and 2,5-dimethoxy-4-iodoamphetamine [DOI] hydrochloride) on EMF-induced analgesia. In total, 66 adult male Wistar albino rats with an average body mass of 225 ± 13 g were used in this study. The animals were subjected to repeated exposures of alternating 50 Hz and 5 mT EMF for 2 h a day for 15 days. Prior to analgesia tests, serotonin HCl (5-HT1 agonist) 4 mg/kg, WAY 100635 (5-HT1 antagonist) 0.04 mg/kg, DOI hydrochloride (5-HT2 receptor agonist) 4 mg/kg, and SB 204741 (5-HT2 antagonist) 0.5 mg/kg doses were injected into rats. For statistical analysis of the data, analysis of variance was used and multiple comparisons were determined by Tukey's test. Administration of serotonin HCl MF (5 mT)-exposed rats produced a significant increase in percent maximal possible effect (% MPE) as compared with EMF group (P < 0.05). On the contrary, injection of WAY 100635 to MF-exposed rats produced a significant decrease in analgesic activity (P < 0.05). Similarly, the administration of DOI hydrochloride significantly increased % MPE values as compared with the EMF group while SB 204741 reduced it (P < 0.05). In conclusion, our results suggested that serotonin 5-HT1 and 5-HT2 receptors play an important role in EMF-induced analgesia; however, further research studies are necessary to understand the mechanism. Bioelectromagnetics. 2019;40:319-330. © 2019 Bioelectromagnetics Society.

中文翻译：

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5-HT1和5-HT 2受体激动剂对大鼠电磁场镇痛的作用。

许多证据证明了磁场（MF）的抗伤害感受作用。但是，电磁场（EMF）的镇痛作用机理尚未完全了解。本研究的目的是研究5-HT1和5-HT2受体激动剂（盐酸5-羟色胺和2,5-二甲氧基-4-碘安非他明[DOI]盐酸盐）对EMF诱导的镇痛作用的影响。总共使用了66只平均体重为225±13 g的成年雄性Wistar白化病大鼠。使动物经受交替的交替的50Hz和5mT EMF暴露2天，持续15天。在进行镇痛测试之前，应使用盐酸羟色胺（5-HT1激动剂）4 mg / kg，WAY 100635（5-HT1拮抗剂）0.04 mg / kg，盐酸DOI（5-HT2受体激动剂）4 mg / kg和SB 204741（5 -HT2拮抗剂）将0.5mg / kg剂量注射到大鼠中。对于数据的统计分析，使用了方差分析，并通过Tukey检验确定了多个比较。与EMF组相比，施用5-羟色胺HCl MF（5 mT）的大鼠产生的最大可能作用百分比（MPE）显着增加（P <0.05）。相反，向暴露于MF的大鼠注射WAY 100635会导致镇痛活性显着降低（P <0.05）。同样，与EMF组相比，DOI盐酸盐的给药显着提高了MPE％值，而SB 204741降低了MPE％（P <0.05）。总之，我们的结果表明5-羟色胺5-HT1和5-HT2受体在EMF诱导的镇痛中起重要作用。但是，有必要进一步研究以了解其机理。生物电磁学。2019; 40：319-330。