The ability of several chelating mycotoxins to form coordination complexes with the lanthanide metals europium and terbium was explored. The mycotoxins examined included ochratoxin A, citrinin, cyclopiazonic acid (CPA), kojic acid, and tenuazonic acid (TeA). Of these compounds, TeA and CPA resulted in the greatest luminescence. Parameters influencing luminescence of TeA were investigated further. These included the type of lanthanide and its concentration, certain environmental factors, and the effect of competing metal cations. Of the two lanthanide metals, the terbium coordination complex (TeA-Tb³⁺) showed greater luminescence relative to the europium complex (TeA-Eu³⁺). The effects of solvent type, water content, and pH on the TeA-Tb³⁺ system suggested that optimal conditions for luminescence were in 90% methanol with 10% aqueous buffer at pH 3. In competitive assays, the luminescence of the TeA-Tb³⁺ complex decreased as the concentration of competing metal cations increased. Among the cations tested, Cu²⁺ was the best inhibitor followed by Al³⁺, Au³⁺, Fe³⁺, Co²⁺, Mn²⁺, Mg²⁺, and Ca²⁺. Two cations, Na⁺ and K⁺, showed no significant inhibition. This is the first report to describe the coordination of the metal-chelating mycotoxin TeA with lanthanides and the ability of TeA to serve as an “antenna” for the efficient transfer of energy to the lanthanide with resulting luminescence. Understanding the ability of mycotoxins such as TeA to chelate metals provides insight into how they exert their toxic effects.

中文翻译：

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发光复合物中霉菌毒素与镧系元素的配位。

探索了几种螯合真菌毒素与镧系金属euro和ter形成配位络合物的能力。检查的霉菌毒素包括曲霉毒素A，柑桔素，环吡唑酸（CPA），曲酸和Tenuazonic酸（TeA）。在这些化合物中，TeA和CPA导致最大的发光。进一步研究了影响TeA发光的参数。这些因素包括镧系元素的类型及其浓度，某些环境因素以及竞争性金属阳离子的影响。在两种镧系金属中，coordination配位配合物（TeA-Tb ³⁺）相对于the配合物（TeA-Eu ³⁺）表现出更大的发光。溶剂类型，含水量和pH对TeA-Tb ^3+的影响该系统表明发光的最佳条件是在90％甲醇和10％pH值为10的水性缓冲液中进行。在竞争性测定中，TeA-Tb ³⁺复合物的发光随着竞争性金属阳离子浓度的增加而降低。在测试的阳离子中，Cu ²⁺是最好的抑制剂，其次是Al ³⁺，Au ³⁺，Fe ³⁺，Co 2 ⁺，Mn 2 ⁺，Mg ²⁺和Ca ²⁺。两个阳离子Na ⁺和K ⁺，无明显抑制作用。这是第一份描述金属螯合霉菌毒素TeA与镧系元素的配合以及TeA用作“天线”以将能量有效转移至镧系元素并产生发光的能力的报告。了解诸如TeA之类的霉菌毒素螯合金属的能力可以洞悉它们如何发挥其毒性作用。