We present ampliCan, an analysis tool for genome editing that unites highly precise quantification and visualization of genuine genome editing events. ampliCan features nuclease-optimized alignments, filtering of experimental artifacts, event-specific normalization, and off-target read detection and quantifies insertions, deletions, HDR repair, as well as targeted base editing. It is scalable to thousands of amplicon sequencing-based experiments from any genome editing experiment, including CRISPR. It enables automated integration of controls and accounts for biases at every step of the analysis. We benchmarked ampliCan on both real and simulated data sets against other leading tools, demonstrating that it outperformed all in the face of common confounding factors.

中文翻译：

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使用ampliCan准确分析真正的CRISPR编辑事件。

我们介绍了ampliCan，这是一种用于基因组编辑的分析工具，它将真正的基因组编辑事件的高精度量化和可视化结合在一起。ampliCan具有核酸酶优化的比对，实验伪影的过滤，事件特定的归一化和脱靶读取检测功能，并可以量化插入，缺失，HDR修复以及目标碱基编辑。它可以扩展到来自任何基因组编辑实验（包括CRISPR）的数千个基于扩增子测序的实验。它可以实现控件的自动集成，并在分析的每个步骤中解决偏差。我们将ampliCan在真实数据集和模拟数据集上与其他领先工具进行了基准比较，证明在面对常见的混杂因素时，它的表现优于所有工具。