A polysaccharide of Irpex lacteus, a white-rot fungus with lignocellulose-degrading activities, has been used as a commercial medicine for nephritis treatment. Previously, a low-intensity electromagnetic field (LI-EMF) was found to increase the biomass and polysaccharide content of Irpex lacteus and induce twists on the cell surface. In this study, RNA-sequencing (RNA-seq) technology was used to analyze the underlying mechanism of LI-EMF's influence on Irpex lacteus. We identified 3268, 1377, and 941 differentially expressed genes (DEGs) in the LI-EMF-treated samples at recovery times of 0 h, 3 h, and 6 h, respectively, indicating a significant decline in the influence of the LI-EMF treatment on Irpex lacteus with the passage of recovery time. Moreover, 30 upregulated and 14 downregulated DEGs overlapped in the LI-EMF-treated samples at the recovery times of 0 h, 3 h, and 6 h, implying the important lasting effects of LI-EMF. The reliability of the RNA-seq data were validated by quantitative real-time PCR (qRT-PCR). The DEGs related to transcription factors, cell proliferation, cell wall, membrane components, amino acid biosynthesis and metabolism, and polysaccharide biosynthesis and metabolism were significantly enriched in the LI-EMF-treated samples. The experiments confirmed that the LI-EMF treatment significantly increased the content of amino acids with a considerable increase in the content of essential amino acids. Therefore, the global gene expression changes explained the pleiotropic effects of Irpex lacteus induced by the LI-EMF treatment. These findings provide the requisite data for the appropriate design and application of LI-EMF in the fermentation of microorganisms to increase production. Bioelectromagnetics. 40:104-117, 2019. © 2019 Bioelectromagnetics Society.

中文翻译：

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全球基因表达变化反映了低强度电磁场诱导的Irpex lacteus的多效性作用。

具有木素纤维素降解活性的白腐真菌——Irpex lacteus的多糖已被用作治疗肾炎的商业药物。以前，发现低强度电磁场（LI-EMF）会增加Irpex lacteus的生物量和多糖含量，并引起细胞表面扭曲。在这项研究中，使用RNA测序（RNA-seq）技术来分析LI-EMF对Irpex lacteus影响的潜在机制。我们在LI-EMF处理的样品中分别在0 h，3 h和6 h的恢复时间鉴定了3268、1377和941个差异表达基因（DEG），表明LI-EMF的影响显着下降随着恢复时间的流逝，对Irpex lacteus进行治疗。此外，在LI-EMF处理的样品中，有30个上调的DEG和14个下调的DEG在0 h，3 h和6 h的恢复时间重叠，这表明LI-EMF具有重要的持久作用。RNA-seq数据的可靠性通过定量实时PCR（qRT-PCR）进行了验证。在LI-EMF处理的样品中，与转录因子，细胞增殖，细胞壁，膜成分，氨基酸生物合成和代谢以及多糖生物合成和代谢相关的DEG显着丰富。实验证实，LI-EMF处理显着增加了氨基酸的含量，而必需氨基酸的含量却显着增加。因此，整体基因表达的变化解释了LI-EMF处理引起的乳酸菌Irpex的多效性作用。这些发现为LI-EMF在微生物发酵中增加产量的适当设计和应用提供了必要的数据。生物电磁学。40：104-117，2019.©2019生物电磁学会。