The histone H3 lysine 4 demethylase RBP2 contains a DNA binding domain, the AT-rich interaction domain (ARID). We solved the structure of ARID by NMR, identified its DNA binding motif (CCGCCC) and characterized the binding contacts. Immunofluorescence and luciferase assays indicated that ARID is required for RBP2 demethylase activity in cells and that DNA recognition is essential to regulate transcription.

中文翻译：

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H3K4脱甲基酶RBP2的ARID结构域与DNA CCGCCC基序结合。

组蛋白H3赖氨酸4脱甲基酶RBP2包含一个DNA结合域，即富含AT的相互作用域（ARID）。我们通过NMR解决了ARID的结构，确定了其DNA结合基序（CCGCCC）并表征了结合接触。免疫荧光和荧光素酶测定表明，ARID是细胞中RBP2脱甲基酶活性所必需的，DNA识别对于调节转录至关重要。