Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8) is the likely infectious cause of Kaposi's sarcoma, primary effusion lymphoma, and some cases of multicentric Castleman's disease. Its latent nuclear antigen (LANA) is expressed in the nuclei of latently infected cells and may play a role in the persistence of episomal viral DNA in dividing cells. Here we report that LANA interacts with RING3, a nuclear protein and member of the Drosophila fsh (female sterile homeotic) family of proteins, some of which have previously been implicated in controlling gene expression. Binding of RING3 to LANA involves the ET domain, characteristic of fsh-related proteins, suggesting that this highly conserved region is involved in protein-protein interactions. The interaction between RING3 and LANA results in phosphorylation of serine and threonine residues located between amino acids 951 and 1107 in the carboxy-terminal region of LANA. However, RING3 is not itself a kinase but appears to recruit an as yet unidentified serine/threonine protein kinase into the complex which it forms with LANA.

中文翻译：

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卡波西氏肉瘤相关疱疹病毒的潜伏核抗原与RING3相互作用，RING3是果蝇雌性不育同源（fsh）基因的同源物。

卡波西氏肉瘤相关疱疹病毒（KSHV / HHV-8）可能是卡波西氏肉瘤，原发渗出性淋巴瘤以及某些多中心卡斯尔曼病的传染病。它的潜伏核抗原（LANA）在潜伏感染细胞的核中表达，并可能在游离细胞中持续存在的游离病毒DNA中起作用。在这里，我们报道LANA与RING3相互作用，该蛋白是一种核蛋白，是果蝇fsh（女性不育同种异体）蛋白家族的成员，其中某些以前与控制基因表达有关。RING3与LANA的结合涉及fsh相关蛋白的特征性ET域，这表明该高度保守的区域参与了蛋白-蛋白相互作用。RING3和LANA之间的相互作用导致位于LANA羧基末端区域的氨基酸951和1107之间的丝氨酸和苏氨酸残基的磷酸化。但是，RING3本身并不是激酶，而是似乎将尚未鉴定的丝氨酸/苏氨酸蛋白激酶募集到与LANA形成的复合物中。