This study aimed to evaluate the potential pathogenicity and antibiotic resistance of 31 environmental Vibrio isolates obtained from surface water in southern and eastern Slovakia. Isolates were identified as Vibrio cholerae non-O1/non-O139 and Vibrio metschnikovii by biochemical tests, MALDI biotyping, and 16S RNA gene sequencing. Analysis of the susceptibility to 13 antibacterial agents showed susceptibility of all isolates to ciprofloxacin, trimethoprim/sulfamethoxazole, chloramphenicol, gentamicin, imipenem, tetracyclin, and doxycycline. We recorded high rates of resistance to β-lactams and streptomycin. Investigation of antibiotic resistance showed five different antibiotic profiles with resistance to antibacterials from three classes, but no multidrug resistance was observed. The investigation of the pathogenic potential of V. cholerae isolates showed that neither the cholera toxin coding gene ctxA nor the genes zot (zonula occludens toxin), ace (accessory cholera toxin), and tcpA (toxin-coregulated pilus) were present in any of 31 isolated samples. Gene ompU (outer membrane protein) was confirmed in 80% and central regulatory protein-coding gene toxR in 71% of V. cholerae isolates, respectively. A high prevalence of the hemolysin coding gene hlyA in all V. cholerae was observed. The data point toward the importance of systematic monitoring and comparative studies of potentially pathogenic vibrios in European countries.

中文翻译：

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斯洛伐克淡水水生弧菌分离株的潜在致病性和抗生素抗性。

这项研究旨在评估从斯洛伐克南部和东部地表水中获得的31种环境弧菌分离物的潜在致病性和抗生素抗性。通过生化测试，MALDI生物分型和16S RNA基因测序，将分离物鉴定为霍乱弧菌非O1 /非O139和梅氏弧菌。对13种抗菌剂的敏感性分析表明，所有分离物对环丙沙星，甲氧苄啶/磺胺甲恶唑，氯霉素，庆大霉素，亚胺培南，四环素和强力霉素的敏感性。我们记录了对β-内酰胺类和链霉素的高耐药率。对抗生素耐药性的研究表明，五种不同的抗生素谱对三类抗生素具有耐药性，但未观察到多药耐药性。V的致病潜力的研究。霍乱杆菌分离株表明，在31个分离的样品中，均未出现霍乱毒素编码基因ctxA或zot（小带闭塞毒素），ace（辅助霍乱毒素）和tcpA（毒素成核的菌毛）基因。在霍乱弧菌分离株中分别有80％和中央调节蛋白编码基因toxR证实了基因ompU（外膜蛋白）。在所有霍乱弧菌中均观察到溶血素编码基因hlyA的高流行。数据表明在欧洲国家中对潜在病原性弧菌进行系统监测和比较研究的重要性。在霍乱弧菌分离株中分别有80％和中央调节蛋白编码基因toxR证实了基因ompU（外膜蛋白）。在所有霍乱弧菌中均观察到溶血素编码基因hlyA的高流行。数据表明在欧洲国家中对潜在病原性弧菌进行系统监测和比较研究的重要性。在霍乱弧菌分离株中分别有80％和中央调节蛋白编码基因toxR证实了基因ompU（外膜蛋白）。在所有霍乱弧菌中均观察到溶血素编码基因hlyA的高流行。数据表明在欧洲国家中对潜在病原性弧菌进行系统监测和比较研究的重要性。