Sogatella furcifera (Horváth), a prominent rice pest in Asia, is a typical R-strategic and highly adaptable insect. Heat shock proteins (Hsps) are highly conserved molecular chaperones regulating responses to various abiotic stresses; however, limited information is available regarding their role in responding to abiotic stress in S. furcifera. This study aimed to investigate the effect of abiotic stresses on the expression of Hsp70 genes in the S. furcifera. Five Hsp70 genes were isolated from S. furcifera, and the expression patterns at different developmental stages and temperatures, upon treatment with different insecticides and ultraviolet A (UV-A) stress, were analyzed. Hsp70 genes were expressed at different developmental stages. Hsp70-2, Hsp70-5, and Hsp70-6 were significantly upregulated upon heat shock at 40 °C for 30 min. Hsp70-3 and Hsp70-4 were significantly upregulated upon heat shock at 30 °C for 30 min. Under UV-A stress, Hsp70-3, Hsp70-4, Hsp70-5, and Hsp70-6 were significantly upregulated. Conversely, Hsp70-2 was significantly downregulated under UV-A stress. The five Hsp70 genes were significantly downregulated in 3rd-instar nymphs on exposure to thiamethoxam, buprofezin, and avermectin at LC₁₀ and LC₂₅ concentrations. Hence, Hsp70 genes significantly contribute to the tolerance of S. furcifera to temperature and UV-A stress; however, they are not involved in the response to insecticides.

中文翻译：

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非生物胁迫对白背飞虱 (Horváth) Hsp70 基因表达的影响。


白背飞虱(Horváth) 是亚洲重要的水稻害虫，是典型的 R 战略性、适应性强的昆虫。热休克蛋白（Hsps）是高度保守的分子伴侣，调节对各种非生物胁迫的反应；然而，关于它们在白背飞虱应对非生物胁迫中的作用的信息有限。本研究旨在探讨非生物胁迫对白背飞虱Hsp70基因表达的影响。从白背飞虱中分离得到5个Hsp70基因，并分析其在不同发育阶段、不同温度、不同杀虫剂处理和紫外线A（UV-A）胁迫下的表达模式。 Hsp70基因在不同发育阶段表达。 40°C 热激 30 分钟后， Hsp70-2 、 Hsp70-5和Hsp70-6显着上调。 30°C 热激 30 分钟后， Hsp70-3和Hsp70-4显着上调。在UV-A胁迫下， Hsp70-3 、 Hsp70-4 、 Hsp70-5和Hsp70-6显着上调。相反， Hsp70-2在 UV-A 胁迫下显着下调。接触 LC ₁₀和 LC ₂₅浓度的噻虫嗪、噻嗪酮和阿维菌素后，三龄若虫的 5 个Hsp70基因显着下调。因此， Hsp70基因显着促进白背飞虱对温度和 UV-A 胁迫的耐受性；然而，它们不参与对杀虫剂的反应。