Many important vaccines use bacterial capsular polysaccharides, or shorter polysaccharides or oligosaccharides, derived from the capsular polysaccharides, conjugated to protein. It is imperative that manufacturers understand the carbohydrate composition of these vaccines and deliver a product with a consistent polysaccharide or polysaccharide conjugate composition and content. High-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) is a major technique used to understand the carbohydrate composition of these vaccines and ensure product quality. HPAE-PAD separates and detects carbohydrates without analyte derivatization. This paper describes the basics of the HPAE-PAD technique and then reviews how it has been applied to Haemophilus influenzae type b, pneumococcal, meningococcal, group B streptococcal, and Salmonella polysaccharide and corresponding conjugate vaccines.

中文翻译：

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使用脉冲安培检测的高性能阴离子交换色谱法确保疫苗质量。

许多重要的疫苗使用细菌荚膜多糖，或更短的多糖或寡糖，衍生自荚膜多糖，与蛋白质结合。制造商必须了解这些疫苗的碳水化合物成分，并提供具有一致多糖或多糖缀合物成分和含量的产品。带脉冲安培检测的高效阴离子交换色谱 (HPAE-PAD) 是用于了解这些疫苗的碳水化合物成分并确保产品质量的主要技术。HPAE-PAD 无需分析物衍生即可分离和检测碳水化合物。本文介绍了 HPAE-PAD 技术的基础知识，然后回顾了它如何应用于 b 型流感嗜血杆菌、肺炎球菌、脑膜炎球菌、B 组链球菌、