BACKGROUND Microwave-induced thermoacoustic tomography (TAT) has potential for detecting germinal matrix hemorrhage (GMH). However, it has not been demonstrated in vivo. OBJECTIVE To demonstrate the feasibility of TAT for in vivo detecting GMH by using neonatal mouse. METHODS A cylindrical-scanning TAT system was developed with optimized microwave irradiation and ultrasound detection for neonatal mouse imaging. Neonatal mice were used to develop GMH model by injection of autologous blood into the periventricular region. After TAT experiments, the animals were sacrificed, frozen and excised to validate the TAT findings. The detailed comparative analyses of the TAT images and corresponding photographs of the excised brain tissues were conducted. RESULTS Satisfactory matches are identified between the TAT images and corresponding histological sections, in terms of the shape and size of the brain tissues. Some organs and tissues were also identified. Particularly, comparing to the corresponding histological sections, using TAT enables to more accurately detect the hematoma region at different depths in the neonatal mouse brain. CONCLUSIONS This study demonstrates for the first time that TAT can detect GMH in neonatal mouse cerebrum in vivo. This represents the first important step towards the in vivo diagnosis and grading of hemorrhage in the infant human brain.

中文翻译：

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新生小鼠大脑生胚基质出血的热声层析成像。

背景技术微波诱导的热声层析成像（TAT）具有检测生发性基质出血（GMH）的潜力。但是，尚未在体内证明。目的证明TAT在新生小鼠体内检测GMH的可行性。方法开发了具有最佳微波辐射和超声检测能力的圆柱扫描TAT系统，用于新生儿小鼠成像。通过将自体血注入脑室周围区域，将新生小鼠用于建立GMH模型。经过TAT实验后，将动物处死，冷冻并切除以验证TAT结果。对TAT图像和切除的脑组织的相应照片进行了详细的比较分析。结果根据脑组织的形状和大小，在TAT图像和相应的组织学切片之间确定了令人满意的匹配。还鉴定了一些器官和组织。特别地，与相应的组织学切片相比，使用TAT能够更准确地检测新生小鼠大脑不同深度的血肿区域。结论这项研究首次证明TAT可以在体内检测新生小鼠大脑中的GMH。这代表了对婴儿人脑的体内诊断和出血分级的重要的第一步。使用TAT可以更准确地检测新生小鼠大脑不同深度的血肿区域。结论这项研究首次证明TAT可以在体内检测新生小鼠大脑中的GMH。这代表了对婴儿人脑的体内诊断和出血分级的重要的第一步。使用TAT可以更准确地检测新生小鼠大脑不同深度的血肿区域。结论这项研究首次证明TAT可以在体内检测新生小鼠大脑中的GMH。这代表了对婴儿人脑的体内诊断和出血分级的重要的第一步。