BACKGROUND We have developed a technology to electrically polarize living bone. OBJECTIVE The effects of stored electrical charge in electrical polarized bone on the facilitation of new bone formation were assayed. METHODS Stimulated depolarized current measurement was performed in electrically polarized and nonpolarized femora of SD rats. These bone specimens were implanted into bone defects of the rat femora and fixed with a custom-made external fixator. X-ray imaging of the implant was performed every week. After 3 weeks, micro-CT scanning was performed to evaluate the displacement rate. Histological observation was performed, and the occupancy ratio of the newly formed bone was calculated from tissue specimens stained with Villanueva's Goldner method. RESULTS There was a tendency for the displacement rate of the implant to be smaller and the occupancy ratio of the newly formed bone to be larger, especially at the distal end, in the polarized group compared with the nonpolarized group. The time of callus appearance was significantly earlier in the polarized group than in the nonpolarized group, and bridging callus grew from the distal to the proximal end. CONCLUSIONS Bone specimens can be electrically polarized, and the stored electrical charge can work effectively to facilitate new bone formation.

中文翻译：

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使用定制的外部固定器对大鼠股骨缺损模型进行电极化的功效。

背景技术我们已经开发出使活骨电极化的技术。目的分析极化电极中储存的电荷对促进新骨形成的影响。方法在SD大鼠的电极化和非极化股骨上进行刺激的去极化电流测量。将这些骨标本植入大鼠股骨的骨缺损中，并用定制的外固定器固定。每周对植入物进行X射线成像。3周后，进行微CT扫描以评估置换率。进行组织学观察，并从用Villanueva的Goldner方法染色的组织样本中计算出新形成的骨头的占有率。结果与非极化组相比，极化组有一种趋势，即植入物的移位率较小，新形成的骨的占有率较大，尤其是在远端。极化组的愈伤组织出现时间明显早于非极化组，并且桥接的愈伤组织从远端生长到近端。结论骨骼标本可以被电极化，并且所存储的电荷可以有效地促进新骨的形成。桥接愈伤组织从远端生长到近端。结论骨骼标本可以被电极化，并且所存储的电荷可以有效地促进新骨的形成。桥接愈伤组织从远端生长到近端。结论骨骼标本可以被电极化，并且所存储的电荷可以有效地促进新骨的形成。