Oxidative stress is initiated by reactive oxygen species, the primary factor in many chronic diseases. Moringa oleifera possesses strong antioxidant properties due to the presence of various phytochemicals. In this study, we investigated the effect of M. oleifera leaf extract on markers of oxidative stress in HL60 cells exposed to oxidative stress. HL60 cells were incubated with different concentrations of M. oleifera leaf extract, and cells were harvested for viability assays on days 1, 2, and 3. Antioxidant indexes (malondialdehyde, reduced glutathione, superoxide dismutase, and catalase) were measured on days 1, 2, and 3. Supplementation with the moringa leaf extract at all concentrations resulted in significant reductions in lipid peroxidation in cells that were or were not incubated in an environment with excess oxidative stress. The most significant reduction in this parameter occurred after 24 h of incubation. The results show that reductions seen in this parameter may be due to the modulation of the endogenous antioxidant defense system by extract supplementation. Cell viability was also improved in cells incubated in moringa leaf extract at concentrations of 800 and 1000 μg/mL. This finding, however, did not corroborate with lipid peroxidation results at 1000 μg/mL extract supplementation. Further investigations are needed to clarify the underlying mechanism responsible for increased cell viability at this concentration. We can, therefore, conclude that the moringa leaf extract offered added protection from oxidative stress within the first 24 h, as well as increasing cell viability at certain concentrations.

中文翻译：

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辣木叶子提取物对遭受氧化应激的人早幼粒细胞白血病细胞的影响。

氧化应激是由活性氧引起的，活性氧是许多慢性疾病的主要因素。由于各种植物化学物质的存在，辣木具有很强的抗氧化性能。在这项研究中，我们调查了油茶树叶提取物对暴露于氧化应激的HL60细胞中氧化应激标志物的影响。HL60细胞与不同浓度的油橄榄分枝杆菌孵育叶提取物，并在第1、2和3天收获细胞用于活力分析。在第1、2和3天测量抗氧化指数（丙二醛，还原型谷胱甘肽，超氧化物歧化酶和过氧化氢酶）。在所有浓度下，无论在有或没有氧化应激的环境中孵育的细胞，脂质过氧化的作用都显着降低。孵育24小时后，该参数的最大降低发生。结果表明，该参数的降低可能是由于提取物补充对内源性抗氧化剂防御系统的调节。细胞存活率也在800和1000浓度辣木叶提取物温育的细胞提高 μ克/毫升。这一发现，但是，并没有与在1000脂质过氧化反应的研究结果证实 μ克/毫升提取补充。需要进一步研究以阐明在该浓度下负责增加细胞生存力的潜在机制。因此，我们可以得出的结论是，辣木叶提取物在最初的24小时内提供了针对氧化应激的额外保护，并在一定浓度下提高了细胞活力。