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Non-small cell lung cancer tumour antigen, MUC-1 peptide-loaded non-aggregated poly (lactide-co-glycolide) nanoparticles augmented cellular uptake in mouse professional antigen-presenting cells: optimisation and characterisation.
Journal of Microencapsulation ( IF 3.0 ) Pub Date : 2019-11-27 , DOI: 10.1080/02652048.2019.1692943
Kiran Jyoti 1, 2 , Sanyog Jain 3 , Om Prakash Katare 4 , Anju Katyal 5 , Ramesh Chandra 5, 6 , Jitender Madan 1
Affiliation  

Aim: MUC-1 lipopeptide vaccine exhibited immense potential in the treatment of non-small cell lung cancer (NSCLC) in both preclinical and clinical trials. However, it lacks triggering of mucosal immunity at the site of action. Therefore, in present investigation, MUC-1 peptide-loaded poly(lactide-co-glycolide) nanoparticles (MUC-1 peptide-PLGA-NPs) and MUC-1 peptide-loaded poly(lactide-co-glycolide) non-aggregated nanoparticles (MUC-1 peptide-PLGA-NA-NPs) using Central Composite Design (CCD) were customised.Methods and Results: The mean particle size of MUC-1 peptide PLGA-NPs was estimated to be 176.7 ± 32.7 nm, significantly (p < 0.05) higher than 100.3 ± 24.3 nm of MUC-1 peptide-PLGA-NA-NPs. Furthermore, integrity and stability of MUC-1 were maintained in MUC-1 peptide PLGA-NA-NPs. MUC-1 peptide-PLGA-NA-NPs exhibited augmented cellular uptake in mouse RAW264.7 macrophages preferably by clathrin-mediated endocytosis pathway as compared to phagocytosis followed by MUC-1-peptide PLGA-NPs owing to size ≤100 nm, and spherical shape.Conclusion: MUC-1 peptide-PLGA-NA-NPs may be a potential candidate to study antitumor potential in xenograft model of NSCLC through inhalation route of administration.

中文翻译:

非小细胞肺癌肿瘤抗原,负载MUC-1肽的非聚集型聚(丙交酯-乙交酯)纳米粒子增加了小鼠专业抗原呈递细胞的细胞摄取:优化和表征。

目的:MUC-1脂肽疫苗在临床前和临床试验中均显示出治疗非小细胞肺癌(NSCLC)的巨大潜力。但是,它在作用部位缺乏触发粘膜免疫的能力。因此,在本研究中,MUC-1肽负载的聚丙交酯-乙交酯共聚物纳米粒子(MUC-1肽-PLGA-NPs)和MUC-1肽负载的聚丙交酯-乙交酯共聚物非聚集纳米粒子方法和结果:MUC-1肽PLGA-NP的平均粒径估计为176.7±32.7 nm,显着(p <0.05)高于100.3±24.3 nm的MUC-1肽-PLGA-NA-NPs。此外,在MUC-1肽PLGA-NA-NP中保持了MUC-1的完整性和稳定性。
更新日期:2019-11-01
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