A novel virus with a (+) single-stranded RNA genome was detected by high-throughput sequencing (HTS) in a sample of grapevine (Vitis vinifera) cv. Kizil Sapak (sample/isolate 127) that originated from Turkmenistan. The complete genome of the virus, tentatively named "grapevine Kizil Sapak virus" (GKSV), is 7,604 nucleotides in length, excluding the poly(A) tail. The genome organization of GKSV, encoded genes, and sequence domains are typical for members of the family Betaflexiviridae, specifically those belonging to the subfamily Trivirinae. Phylogenetic analysis placed GKSV within the subfamily Trivirinae, in the same clade as fig latent virus 1 (FLV-1) but distinct from the clades formed by members of other genera. A comparative analysis of GKSV-127 with the HTS-derived sequences obtained from two additional isolates showed that they are genetic variants of the same virus species. Based on current ICTV species and genus demarcation criteria, and the results of the sequence and phylogenetic analyses, we propose that GKSV and FLV-1 represent a new genus within the subfamily Trivirinae.

中文翻译：

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葡萄科克西尔Sapak病毒的鉴定和基因组学表征，这是Betaflexiviridae家族的新型葡萄感染成员。

通过高通量测序（HTS）在葡萄（Vitis vinifera）cv样品中检测到具有（+）单链RNA基因组的新型病毒。源自土库曼斯坦的Kizil Sapak（样本/分离物127）。该病毒的完整基因组，暂定名为“葡萄树Kizil Sapak病毒”（GKSV），长度为7,604个核苷酸，不包括poly（A）尾巴。GKSV的基因组组织，编码的基因和序列域是Betaflexiviridae家族成员（特别是属于Trivirinae亚科的成员）的典型成员。系统发育分析将GKSV置于Trivirinae亚科中，与无花果潜伏病毒1（FLV-1）位于同一进化枝，但与其他属成员形成的进化枝不同。GKSV-127与从另外两个分离物中获得的HTS衍生序列的比较分析表明，它们是同一病毒物种的遗传变异。根据当前的ICTV种类和属划分标准，以及序列和系统发育分析的结果，我们建议GKSV和FLV-1代表Trivirinae亚科中的一个新属。