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Authentication Analysis of MT-4 Cells Distributed by the National Institutes of Health AIDS Reagent Program.
Journal of Virology ( IF 5.4 ) Pub Date : 2019-11-26 , DOI: 10.1128/jvi.01390-19
Melissa V Fernandez 1 , Krista A Delviks-Frankenberry 2 , David A Scheiblin 3 , Christine Happel 4 , Vinay K Pathak 2 , Eric O Freed 5
Affiliation  

The MT-4 human T-cell line expresses HTLV-1 Tax and is permissive for replication of an HIV-1 gp41 mutant lacking the cytoplasmic tail. MT-4 cells (lot 150048), distributed by the NIH AIDS Reagent Program (NIH-ARP), were found to be Tax deficient and unable to host replication of the gp41-truncated HIV-1 mutant. These findings, together with short tandem repeat profiling, established that lot 150048 are not bona fide MT-4 cells.

中文翻译:

美国国立卫生研究院艾滋病试剂计划分发的MT-4细胞的鉴定分析。

MT-4人类T细胞系表达HTLV-1税收,并且允许复制缺少细胞质尾巴的HIV-1 gp41突变体。NIH AIDS试剂计划(NIH-ARP)分发的MT-4细胞(批次150048)被发现缺乏税,并且无法宿主gp41截短的HIV-1突变体的复制。这些发现以及较短的串联重复序列分析证明,批次150048不是真正的MT-4细胞。
更新日期:2019-11-01
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