Human-induced pluripotent stem cells (hiPSCs) have shown great potential toward practical and scientific applications. We previously reported the generation of human dental pulp stem cells using non-integrating replication-defective Sendai virus (SeVdp) vector in feeder-free culture with serum-free medium hESF9. This study describes the generation of hiPSCs from peripheral blood mononuclear cells to increase the donor population, while reducing biopsy invasiveness. From 6-d-old primary culture of peripheral blood mononuclear cells (PBMCs) with IL-2, hiPSCs were established using SeVdp(KOSM)302L with recombinant Laminin-511 E8 fragments under serum-free condition. The established PBMC-derived hiPSCs showed pluripotency and differentiation ability both in vivo and in vitro. In addition, we evaluated microarray data from PBMC- and dental pulp-derived hiPSCs. These hiPSCs will be beneficial for characterizing the molecular mechanisms of cellular differentiation and may provide useful substrates for developing cellular therapeutics.

中文翻译：

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在无血清和无饲养层的条件下诱导无整合人类诱导的多能干细胞。

人类诱导的多能干细胞（hiPSC）在实际和科学应用中显示出巨大潜力。我们以前曾报道过，在无血清培养基hESF9的无饲养层培养中，使用非整合型复制缺陷型仙台病毒（SeVdp）载体可产生人牙髓干细胞。这项研究描述了从外周血单个核细胞中产生hiPSC，以增加供体群体，同时减少活检侵入性。从具有IL-2的6 d外周血单个核细胞（PBMC）的原代培养物中，使用SeVdp（KOSM）302L与重组Laminin-511 E8片段在无血清条件下建立hiPSC。建立的PBMC来源的hiPSC在体内和体外均显示出多能性和分化能力。此外，我们评估了来自PBMC和牙髓衍生的hiPSC的微阵列数据。这些hiPSCs对于表征细胞分化的分子机制将是有益的，并可为开发细胞疗法提供有用的底物。