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Induction of integration-free human-induced pluripotent stem cells under serum- and feeder-free conditions.
In Vitro Cellular & Developmental Biology - Animal ( IF 1.665 ) Pub Date : 2019-11-25 , DOI: 10.1007/s11626-019-00412-w
Atsuko Hamada,Eri Akagi,Sachiko Yamasaki,Hirotaka Nakatao,Fumitaka Obayashi,Manami Ohtaka,Ken Nishimura,Mahito Nakanishi,Shigeaki Toratani,Tetsuji Okamoto

Human-induced pluripotent stem cells (hiPSCs) have shown great potential toward practical and scientific applications. We previously reported the generation of human dental pulp stem cells using non-integrating replication-defective Sendai virus (SeVdp) vector in feeder-free culture with serum-free medium hESF9. This study describes the generation of hiPSCs from peripheral blood mononuclear cells to increase the donor population, while reducing biopsy invasiveness. From 6-d-old primary culture of peripheral blood mononuclear cells (PBMCs) with IL-2, hiPSCs were established using SeVdp(KOSM)302L with recombinant Laminin-511 E8 fragments under serum-free condition. The established PBMC-derived hiPSCs showed pluripotency and differentiation ability both in vivo and in vitro. In addition, we evaluated microarray data from PBMC- and dental pulp-derived hiPSCs. These hiPSCs will be beneficial for characterizing the molecular mechanisms of cellular differentiation and may provide useful substrates for developing cellular therapeutics.
更新日期:2019-11-01

 

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