This study investigated the synthesis of 2-ethylhexyl oleate catalyzed by Candida antarctica lipase immobilized on magnetic poly(styrene-co-divinylbenzene) particles in a continuous packed-bed bioreactor. Runs were carried out in a solvent-free system at 50 °C. The performance of the reactor was evaluated for substrates composed by oleic acid and 2-ethylhexanol at five molar ratios (1:4-4:1), determining its operation limits in terms of substrate flow rate. The system performance was quantified for three different flow rates corresponding to space-time between 3 and 12 h. For each condition, the influence of the space-time in the ester formation, esterification yield and productivity was determined. The molar ratio of acid-to-alcohol interfered, in a remarkable way, in the formation of 2-ethylhexyl oleate and the best performance was attained for substrate at equimolar ratio running at 12 h space-time. Under this condition, average 2-ethylhexyl oleate concentration was 471.65 ± 2.98 g L-1 which corresponded to ester productivity of 23.16 ± 0.49 mmol g-1 L-1 h-1. This strategy also gave high biocatalyst operational stability, revealing a half-life time of 2063 h. A model based on the ping-pong Bi-Bi mechanism was developed to describe the kinetics of the esterification reaction and validated using experimental data. The goodness of fit of the model was satisfactory (R2 = 0.9310-0.9952).

中文翻译：

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固定在磁性聚合物载体上的南极假丝酵母脂肪酶连续流动催化合成油酸2-乙基己酯。

这项研究研究了固定在磁性聚（苯乙烯-co-二乙烯基苯）颗粒在连续填充床生物反应器中的南极假丝酵母脂肪酶催化的油酸2-乙基己酯的合成。在无溶剂的系统中于50°C下运行。评价了由油酸和2-乙基己醇以五个摩尔比（1：4-4：1）组成的底物的反应器性能，确定了根据底物流速的操作极限。针对三种不同的流速（对应于3到12小时之间的时空）对系统性能进行了量化。对于每种条件，确定了时空对酯形成，酯化收率和生产率的影响。酸与醇的摩尔比显着地影响了 在油酸2-乙基己酯的形成过程中，当等摩尔比的底物在12 h时空运行时，可获得最佳性能。在此条件下，油酸2-乙基己基酯的平均浓度为471.65±2.98 g L-1，相当于酯产率为23.16±0.49 mmol g-1 L-1 h-1。该策略还提供了高生物催化剂操作稳定性，显示了2063小时的半衰期。建立了基于乒乓球Bi-Bi机制的模型来描述酯化反应的动力学，并使用实验数据进行了验证。模型的拟合度令人满意（R2 = 0.9310-0.9952）。该策略还提供了高生物催化剂操作稳定性，显示了2063小时的半衰期。建立了基于乒乓球Bi-Bi机制的模型来描述酯化反应的动力学，并使用实验数据进行了验证。模型的拟合度令人满意（R2 = 0.9310-0.9952）。该策略还提供了高生物催化剂操作稳定性，显示了2063小时的半衰期。建立了基于乒乓球Bi-Bi机制的模型来描述酯化反应的动力学，并使用实验数据进行了验证。模型的拟合度令人满意（R2 = 0.9310-0.9952）。