Purpose: In rodents, dextran sulfate sodium (DSS)-induced diarrhea and colonic inflammation have similar symptoms to those of ulcerative colitis in humans. We examined the effects of exposure to mild hyperbaric oxygen (MHO) at an atmospheric pressure of 1317 hPa with 40% oxygen on DSS-induced diarrhea and colonic inflammation in rats.
Methods: Five-week-old male Kyoto Apc Delta (KAD) rats (n = 12) were administered 2% DSS through drinking water for 1 week. Subsequently, DSS-treated male rats were not subjected to any further treatment (n = 6) or exposed to MHO (n = 6) for 2 weeks. Age-matched KAD rats not subjected to DSS treatment or exposed to MHO were used as the control group (n = 6).
Results: Control rats did not exhibit diarrhea and colonic inflammation. However, DSS-treated rats exhibited diarrhea and colonic inflammation, regardless of exposure to MHO. Exposure to MHO for 2 weeks led to decreased incidence of diarrhea in DSS-treated rats (p < 0.05). Exposure to MHO had no effect on colonic inflammation in DSS-treated rats (p = 0.12).
Conclusion: Exposure to MHO for 2 weeks can improve diarrhea but cannot attenuate colonic inflammation, possibly due to the short exposure duration (2 weeks) used in this study.

Keywords: colonic inflammation, dextran sulfate sodium, Kyoto Apc Delta rats, mild hyperbaric oxygen, oxidative metabolism


中文翻译：

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暴露于轻度高压氧对 DSS 引起的大鼠结肠炎症和腹泻的影响。

目的：在啮齿动物中，硫酸葡聚糖钠 (DSS) 引起的腹泻和结肠炎症与人类溃疡性结肠炎的症状相似。我们检查了在 1317 hPa 的大气压和 40% 的氧气下暴露于温和高压氧 (MHO) 对 DSS 诱导的大鼠腹泻和结肠炎症的影响。
方法： 5 周大的雄性京都 Apc Delta (KAD) 大鼠 (n = 12) 通过饮用水给予 2% DSS 1 周。随后，DSS 治疗的雄性大鼠未接受任何进一步的治疗 (n = 6) 或暴露于 MHO (n = 6) 2 周。未接受 DSS 治疗或暴露于 MHO 的年龄匹配的 KAD 大鼠用作对照组 (n = 6)。
结果：对照大鼠没有表现出腹泻和结肠炎症。然而，无论是否暴露于 MHO，DSS 治疗的大鼠都表现出腹泻和结肠炎症。暴露于 MHO 2 周导致 DSS 治疗大鼠腹泻发生率降低 ( p < 0.05)。暴露于 MHO 对 DSS 治疗大鼠的结肠炎症没有影响 ( p = 0.12)。
结论：暴露于 MHO 2 周可以改善腹泻，但不能减轻结肠炎症，这可能是由于本研究中使用的暴露时间短（2 周）。

关键词：结肠炎症，硫酸葡聚糖钠，Kyoto Apc Delta 大鼠，轻度高压氧，氧化代谢