Epstein–Barr virus (EBV) associated with several diseases such as contagious mononucleosis chronic active EBV infection, and diverse sorts of malignant tumors. Therefore, using applicable vaccines could be advantageous for public health. Yet, the vaccine has been unavailable to protect from EBV so far. In the current study, to develop a multi-peptide vaccine for EBV and assess its expression in Pichia pastoris yeast system, three immunodominant sequences in glycoprotein (gp) 85, gp350 and latent membrane protein 1 (LMP1) were chosen. To construct fusion peptide, -GGGGS- liker was applied. After cloning the fusion peptide in the pPICZαA expression vector, this recombinant vector processed and transfected into Pichia pastoris host cells. The expression of high level of EBV fusion peptide was confirmed by dot blot and SDS-PAGE procedures. The Pichia pastoris is capable of supporting EBV fusion peptide expression. The application of this fusion peptide as a peptide vaccine to fight EBV is suggested.

Epstein-Barr 病毒 (EBV) 与多种疾病有关，例如传染性单核细胞增多症、慢性活动性 EBV 感染和多种恶性肿瘤。因此，使用适用的疫苗可能有利于公共卫生。然而，到目前为止，还没有疫苗可以预防 EBV。在目前的研究中，为了开发 EBV 多肽疫苗并评估其在毕赤酵母系统中的表达，选择了糖蛋白 (gp) 85、gp350 和潜伏膜蛋白 1 (LMP1) 中的三个免疫显性序列。为了构建融合肽，应用了-GGGGS-liker。在pPICZαA表达载体中克隆融合肽后，该重组载体加工转染毕赤酵母宿主细胞。通过斑点印迹和SDS-PAGE程序证实了高水平的EBV融合肽的表达。在巴斯德毕赤酵母是能够支持EBV融合肽的表达。建议将该融合肽用作肽疫苗来对抗EBV。