BACKGROUND The development of biomaterial scaffolds and implementation of tissue engineering techniques are necessary. Therefore, Polycaprolactone/Sodium Hyaluronate/Multiwalled Carbon Nanotubes/Extract of Mimosa tenuiflora composites have been produced by a thermally-induced phase separation method. OBJECTIVE The objective of this research was to evaluate the in vitro bioactivity and in vitro biocompatibility of the composites. METHODS The in vitro bioactivity of the composites was assessed by soaking them in simulated body fluid for 7, 14, 21, and 28 days. The structure and composition of the composites were analyzed using scanning electron microscopy coupled with energy dispersive spectroscopy and Fourier transform infrared spectroscopy. Also, the in vitro biocompatibility of the composites was evaluated by means of alkaline phosphatase activity of the osteoblasts and by measuring the metabolic activity of the cells using MTT assay. RESULTS The results show a porous and interconnected morphology with enhanced bioactivity. It was observed that the incorporation of Mimosa tenuiflora in the composites promotes increased viability of osteoblasts in the scaffolds. CONCLUSIONS The results show the efficiency of bioactive and biocompatible composites and their potential as candidates for tissue engineering applications.

中文翻译：

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聚己内酯/透明质酸/多壁碳纳米管/提取自含羞草复合材料的体外生物活性和体外生物相容性的评估。

背景技术生物材料支架的开发和组织工程技术的实施是必要的。因此，已经通过热诱导相分离法制备了聚己内酯/透明质酸钠/多壁碳纳米管/含羞草复合材料的提取物。目的本研究的目的是评估复合材料的体外生物活性和体外生物相容性。方法通过将复合材料浸泡在模拟体液中7、14、21和28天来评估其体外生物活性。使用扫描电子显微镜，能量色散光谱和傅里叶变换红外光谱分析复合材料的结构和组成。也，通过成骨细胞的碱性磷酸酶活性并通过使用MTT测定法测量细胞的代谢活性来评估复合物的体外生物相容性。结果结果显示具有增强的生物活性的多孔且相互连接的形态。观察到，含羞草含羞草在复合物中的结合促进了成骨细胞在支架中的生存能力的提高。结论结果表明，生物活性和生物相容性复合材料的效率及其在组织工程应用中的潜力。观察到，含羞草含羞草在复合物中的结合促进了成骨细胞在支架中的生存能力的提高。结论结果表明，生物活性和生物相容性复合材料的效率及其在组织工程应用中的潜力。观察到，含羞草含羞草在复合物中的结合促进了成骨细胞在支架中的生存能力的提高。结论结果表明，生物活性和生物相容性复合材料的效率及其在组织工程应用中的潜力。