BACKGROUND/OBJECTIVE Ischemic heart disease is a major cause of mortality worldwide. Myocardial tissue engineering aims to create transplantable units of myocardium for the treatment of myocardial necrosis caused by ischemic heart disease - bioreactors are used to condition these bioartificial tissues before application. METHODS Our group developed a multimodal bioreactor consisting of a linear drive motor for pulsatile flow generation (500 ml/min) and an external pacemaker for electrical stimulation (10 mA, 3 V at 60 Hz) using LinMot-Talk Software to synchronize these modes of stimulation. Polyurethane scaffolds were seeded with 0.750 × 106 mesenchymal stem cells from umbilical cord tissue per cm2 and stimulated in our system for 72 h, then evaluated. RESULTS After conditioning histology showed that the patches consisted of a cell multilayer surviving stimulation without major damage by the multimodal stimulation, scanning electron microscopy showed a confluent cell layer with no cell-cell interspaces visible. No cell viability issues could be identified via Syto9-Propidium Iodide staining. CONCLUSIONS This bioreactor allows mechanical stimulation via pulsatile flow and electrical stimulation through a pacemaker. Our stem cell-polyurethane constructs displayed survival after conditioning. This system shows feasibility in preliminary tests.

中文翻译：

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多模式刺激生物反应器通过电脉冲和搏动性灌注调节干细胞接种的心脏斑块的可行性研究。

背景/目的缺血性心脏病是世界范围内死亡的主要原因。心肌组织工程旨在创建可移植的心肌单元，以治疗由缺血性心脏病引起的心肌坏死-生物反应器在应用前用于调节这些生物人工组织。方法我们的小组开发了一种多模式生物反应器，该系统包括一个用于产生脉动流的线性驱动电机（500 ml / min）和一个用于电刺激的外部起搏器（10 mA，60 Hz时为3 V），使用LinMot-Talk软件同步这些模式。刺激。每平方厘米从脐带组织中接种0.750×106个间充质干细胞，然后在我们的系统中刺激72小时，然后评估聚氨酯支架。结果在经过组织学检查后发现，该斑块由存活下来的多层细胞组成，而没有受到多模态刺激的严重破坏，扫描电子显微镜显示汇合的细胞层没有可见的细胞间间隙。通过Syto9-碘化丙锭染色无法发现细胞活力问题。结论该生物反应器允许通过脉动流进行机械刺激，并通过起搏器进行电刺激。我们的干细胞-聚氨酯构建物经过调理后显示出存活率。该系统在初步测试中显示出可行性。结论该生物反应器允许通过脉动流进行机械刺激，并通过起搏器进行电刺激。我们的干细胞-聚氨酯构建物经过调理后显示出存活率。该系统在初步测试中显示出可行性。结论该生物反应器允许通过脉动流进行机械刺激，并通过起搏器进行电刺激。我们的干细胞-聚氨酯构建物经过调理后显示出存活率。该系统在初步测试中显示出可行性。