BACKGROUND Calcium polyphosphate (CPP) is a commonly used biomaterial in bone tissue engineering, but CPP is insufficient in osteoinduction. This study aimed to fabricate lithium doped CPP (LiCPP) scaffolds and assess their characterization, degradation, biocompatibility and osteogenesis behavior for bone tissue engineering. METHODS The novel scaffold was characterized by XRD, FTIR and SEM. The porosity, cell mediated degradation behavior and mechanical properties were also investigated. Meanwhile, cell proliferation activity and adhesion in vitro was exploited. Finally, osteogenesis the LiCPP scaffolds in vitro and in vivo was researched. RESULTS The outcomes revealed that low-content Li doping had no significant influence on the structure of CPP. The results of cells mediated degradation experiments from the weight loss and the release of ions indicated that Li doped CPP improved biological degradation. The compressive strength of CPP with 66% porosity was improved to 7 MPa. Cells proliferation experiment and adhesion experiment demonstrated 2.0%LiCPP scaffold was most beneficial to cell growth and attachment. Furthermore, Li doped CPP up-regulated Wnt signal pathway when co-cultured with MG63 and increased osteogenic marker ALP expression and calcium phosphate deposition in vitro. At the same time, new bone formation in vivo was also enhanced by using LiCPP scaffolds and the 2.0%LiCPP scaffolds obtained best osteogenesis outcomes. CONCLUSION The results obtained in our study suggest that 2.0%LiCPP scaffold could benefit from improving the osteogenesis behavior and is a promising biomaterial for bone repairing applications.

中文翻译：

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锂掺杂多磷酸钙钙在骨组织工程中的降解，生物相容性和成骨行为的评估。

背景技术聚磷酸钙（CPP）是骨组织工程中常用的生物材料，但是CPP在骨诱导中不足。这项研究旨在制造掺锂的CPP（LiCPP）支架，并评估其特征，降解，生物相容性和成骨行为，以用于骨组织工程。方法采用XRD，FTIR和SEM对新型支架材料进行表征。还研究了孔隙率，细胞介导的降解行为和机械性能。同时，利用细胞的体外增殖活性和粘附性。最后，研究了LiCPP支架的成骨作用。结果结果表明，低含量的Li掺杂对CPP的结构没有显着影响。由重量损失和离子释放引起的细胞介导的降解实验结果表明，掺Li的CPP改善了生物降解。孔隙率为66％的CPP的抗压强度提高到7 MPa。细胞增殖实验和粘附实验表明2.0％LiCPP支架最有利于细胞生长和附着。此外，当与MG63共培养时，Li掺杂的CPP上调了Wnt信号通路，并在体外增加了成骨标记物ALP表达和磷酸钙沉积。同时，通过使用LiCPP支架还可以增强体内新骨的形成，并且2.0％LiCPP支架获得了最佳的成骨效果。结论我们的研究结果表明2。