This study aims to clarify the prevalence and significance of the emergence of hepatitis B virus (HBV) pre-S/S mutations in children with hepatitis B virus-associated membranous nephropathy (HBVMN). Direct sequencing of polymerase chain reaction products of renal tissue samples that were obtained via percutaneous renal biopsy from seven children revealed the presence of HBV DNA. Seven adr subtypes were analyzed. Deletions in the HBV pre-S region were observed once per seven patients. The deletions were noted in both the pre-S1 (27 bp) and pre-S2 (60 bp) regions. Various point mutations in the HBV pre-S region were detected in all seven patients and proved to be more frequent in the pre-S1 region than in the S2 region. Point mutations in the HBV S region were detected in six patients. Among these mutations, the mutation in the "a" determinant region was noted in five patients. No deletion, however, was observed in the HBV S region. These observations suggested that deletions and point mutations in the HBV pre-S1 and pre-S2 regions and point mutations in the HBV S region, especially the "a" determinant region, are common frequent findings. These results also suggested that HBV pre-S/S region mutations may be involved in the pathogenesis in children with HBVMN.

中文翻译：

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乙型肝炎病毒相关性膜性肾病患儿肾脏组织中乙型肝炎病毒前S / S基因突变的研究。

本研究旨在阐明患乙型肝炎病毒相关性膜性肾病（HBVMN）患儿的乙型肝炎病毒（HBV）pre-S / S突变的发生率和意义。通过对七个孩子的经皮肾脏活检获得的肾脏组织样品的聚合酶链反应产物的直接测序显示了HBV DNA的存在。分析了七个adr亚型。每7名患者观察一次HBV pre-S区域的缺失。在前S1（27 bp）和前S2（60 bp）区域都注意到了缺失。在所有7例患者中均检测到HBV前S区的各种点突变，并证明在S1前区比S2区更频繁。在六名患者中检测到HBV S区的点突变。在这些突变中，“ a”中的突变 在五名患者中发现了决定因素区域。然而，在HBV S区域未观察到缺失。这些观察结果表明，HBV前S1和S2前区的缺失和点突变以及HBV S区（特别是“ a”决定簇区）中的点突变是常见的常见发现。这些结果还表明，HBVN儿童的发病机制可能与HBV前S / S区突变有关。