Our objectives were to evaluate the interaction between host genetics and vaginal microbiota and their relationships with antibody (Ab) response to porcine reproductive and respiratory syndrome virus (PRRSV) vaccination and farrowing performance in commercial gilts. The farrowing performance traits were number born alive, number weaning (NW), total number born, number born dead, stillborn, mummies and preweaning mortality (PWM). The vaginal microbiota was collected on days 4 (D4) and 52 (D52) after vaccination for PRRSV. Blood samples were collected on D52 for Ab measurement. Actinobacteria, Bacterioidetes, Firmicutes, Proteobacteria and Tenericutes were the most abundant Phyla identified in the vaginal microbiota. Heritability ranged from ~0 to 0.60 (Fusobacterium) on D4 and from ~0 to 0.63 (Terrisporobacter) on D52, with 43 operational taxonomic units (OTUs) presenting moderate to high heritability. One major QTL on chromosome 12 was identified for 5 OTUs (Clostridiales, Acinetobacter, Ruminococcaceae, Campylobacter and Anaerococcus), among other 19 QTL. The microbiability for Ab response to PRRSV vaccination was low for both days (<0.07). For farrowing performance, microbiability varied from <0.001 to 0.15 (NW on D4). For NW and PWM, the microbiability was greater than the heritability estimates. Actinobacillus, Streptococcus, Campylobacter, Anaerococcus, Mollicutes, Peptostreptococcus, Treponema and Fusobacterium showed different abundance between low and high Ab responders. Finally, canonical discriminant analyses revealed that vaginal microbiota was able to classify gilts in high and low Ab responders to PRRSV vaccination with a misclassification rate of <0.02. Although the microbiota explained limited variation in Ab response and farrowing performance traits, there is still potential to explore the use of vaginal microbiota to explain variation in traits such as NW and PWM. In addition, these results revealed that there is a partial control of host genetic over vaginal microbiota, suggesting a possibility for genetic selection on the vaginal microbiota.

中文翻译：

---

调查阴道微生物群与宿主遗传学之间的关系及其对商业小母猪免疫反应和分娩性状的影响

我们的目标是评估宿主遗传学和阴道微生物群之间的相互作用，以及它们与对猪繁殖和呼吸综合征病毒 (PRRSV) 疫苗接种的抗体 (Ab) 反应和商业小母猪的分娩性能之间的关系。分娩性能特征是活产数、断奶数 (NW)、总出生数、死产数、死产、木乃伊和断奶前死亡率 (PWM)。在接种 PRRSV 疫苗后第 4 (D4) 天和第 52 (D52) 天收集阴道微生物群。在 D52 收集血样用于 Ab 测量。放线菌、拟杆菌门、厚壁菌门、变形菌门和特尼古菌门是阴道微生物群中最丰富的门。D4 的遗传力范围为 ~0 到 0.60（梭杆菌），D52 的遗传力范围为 ~0 到 0.63（Terrisporobacter），有 43 个操作分类单位 (OTU)，具有中度至高度的遗传力。12 号染色体上的一个主要 QTL 被鉴定为 5 个 OTU（梭状芽孢杆菌、不动杆菌、瘤胃球菌科、弯曲杆菌和厌氧菌），以及其他 19 个 QTL。这两天对 PRRSV 疫苗接种的 Ab 反应的微生物性都很低 (<0.07)。对于分娩性能，微生物率从 <0.001 到 0.15 不等（D4 上的 NW）。对于 NW 和 PWM，微生物性大于遗传力估计值。放线杆菌、链球菌、弯曲杆菌、厌氧球菌、软球菌、消化链球菌、密螺旋体和梭杆菌在低和高 Ab 反应者之间显示出不同的丰度。最后，典型判别分析表明，阴道微生物群能够对 PRRSV 疫苗接种的高和低 Ab 反应者对小母猪进行分类，错误分类率 <0.02。尽管微生物群解释了 Ab 反应和分娩性能特征的有限变化，但仍有潜力探索使用阴道微生物群来解释 NW 和 PWM 等特征的变化。此外，这些结果表明，宿主遗传对阴道微生物群有部分控制，这表明阴道微生物群的遗传选择可能存在。