Composite scaffolds are made by various methods, such as copolymerization, freeze gelation, and thermally induced phase separation, which can compound materials with different properties using solvents and heat. However, it is difficult to compound solvents and heat-sensitive materials such as natural polymers. In this study, we investigated a vacuum pressure impregnation (VPI) method for creating a composite of natural polymers. A collagen solution could not be introduced into a poly (l-lactide) (PLA) porous material using an immersing treatment, but it is possible using the VPI method. The resulting PLA-collagen composite scaffold had greater water adsorption and degradation than a PLA scaffold. These results indicate that VPI may be a promising method for creating composites of natural materials. Impact Statement This is the development of a method for introducing cells into a completed porous material in a short time. This technology is expected to be applied to tissue regeneration and 3D culture.

中文翻译：

---

真空压力浸渍法制备PLA-胶原复合支架。

复合支架是通过多种方法制造的，例如共聚，冷冻凝胶化和热诱导相分离，这些方法可以使用溶剂和热将具有不同性能的材料复合在一起。但是，很难将溶剂和热敏材料（例如天然聚合物）混合在一起。在这项研究中，我们研究了真空压力浸渍（VPI）方法来创建天然聚合物的复合材料。使用浸没处理无法将胶原蛋白溶液引入到聚（l-丙交酯）（PLA）多孔材料中，但是可以使用VPI方法。所得的PLA-胶原复合支架比PLA支架具有更大的水吸附和降解。这些结果表明，VPI可能是创建天然材料复合材料的一种有前途的方法。影响陈述这是一种在短时间内将细胞引入完整的多孔材料中的方法的发展。该技术有望应用于组织再生和3D培养。