Fish cell lines are widely used for the studies of developmental biology, virology, biology of aging, and nutrition physiology. However, little is known about their physicochemical properties. Here, we report the phospholipid compositions and mechanical properties of cell membranes derived from freshwater, anadromous and marine fish species. Biophysical analyses revealed that fish cell lines have highly deformable cell membranes with significantly low membrane tensions and Young's moduli compared with those of mammalian cell lines. The induction of cellular senescence by DNA demethylation using 5-Aza-2'-deoxycytidine significantly reduced the deformability of fish cell membrane, but hydrogen peroxide-induced oxidative stress did not affect the deformability. Mass spectrometry analysis of phospholipids revealed that the level of phosphatidylethanolamine molecules containing polyunsaturated fatty acids significantly increased during the 5-Aza-2'-deoxycytidine-induced cellular senescence. Fish cell lines provide a useful model system for studying the changes in the physicochemical properties of cell membranes during cellular senescence.Abbreviations: 2D-TLC: two-dimensional thin layer chromatography; 5-Aza-dC: 5-Aza-2'-deoxycytidine; DHA: docosahexaenoic acid; EPA: eicosapentaenoic acid; FBS: fetal bovine serum; PC: phosphatidylcholine; PE: phosphatidylethanolamine; PI: phosphatidylinositol; PS: phosphatidylserine; PUFA: polyunsaturated fatty acid; SA-β-gal: senescence-associated beta-galactosidase; SM: sphingomyelin.

中文翻译：

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鱼细胞膜在细胞衰老过程中的理化性质的变化。

鱼细胞系被广泛用于发育生物学，病毒学，衰老生物学和营养生理学的研究。然而，对其理化性质了解甚少。在这里，我们报告了来自淡水，水生和海洋鱼类物种的磷脂成分和细胞膜的机械性能。生物物理分析表明，与哺乳动物细胞系相比，鱼细胞系具有高度可变形的细胞膜，膜张力和杨氏模量显着低。使用5-Aza-2'-脱氧胞苷通过DNA脱甲基作用诱导细胞衰老显着降低了鱼细胞膜的可变形性，但过氧化氢诱导的氧化应激并未影响可变形性。磷脂的质谱分析表明，在5-Aza-2'-脱氧胞苷诱导的细胞衰老过程中，含有多不饱和脂肪酸的磷脂酰乙醇胺分子的水平显着增加。鱼细胞系为研究细胞衰老过程中细胞膜理化性质的变化提供了有用的模型系统。缩写：2D-TLC：二维薄层色谱法；5-Aza-dC：5-Aza-2'-脱氧胞苷；DHA：二十二碳六烯酸；EPA：二十碳五烯酸；FBS：胎牛血清；PC：磷脂酰胆碱；PE：磷脂酰乙醇胺；PI：磷脂酰肌醇；PS：磷脂酰丝氨酸；PUFA：多不饱和脂肪酸；SA-β-gal：衰老相关的β-半乳糖苷酶；SM：鞘磷脂。