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Identification of Reg3β-producing cells using IL-22-stimulated enteroids.
Bioscience, Biotechnology, and Biochemistry ( IF 1.4 ) Pub Date : 2019-11-23 , DOI: 10.1080/09168451.2019.1695575
Mika Sato 1 , Akihiko Inaba 2 , Ken Iwatsuki 2 , Yuki Saito 1 , Miki Tadaishi 1 , Makoto Shimizu 1 , Kazuo Kobayashi-Hattori 1
Affiliation  

Reg3β, a lectin, displays antibacterial activity. This study investigated Reg3β-expressing cells using IL-22-stimulated enteroids. IL-22 stimulation elevated the mRNA and protein levels of Reg3β. IL-22 also increased the mRNA levels of CD133 (a transit-amplifying cell marker) and lysozyme (a Paneth cell marker). Immunohistochemistry showed partial colocalization of Reg3β- and lysozyme-positive cells, suggesting that Paneth cells are one of Reg3β-producing cells.

中文翻译:


使用 IL-22 刺激的肠类鉴定 Reg3β 生成细胞。



Reg3β 是一种凝集素,具有抗菌活性。本研究使用 IL-22 刺激的肠类研究了 Reg3β 表达细胞。 IL-22 刺激提高了 Reg3β 的 mRNA 和蛋白水平。 IL-22 还增加了 CD133(一种转运放大细胞标记物)和溶菌酶(一种潘氏细胞标记物)的 mRNA 水平。免疫组织化学显示 Reg3β 和溶菌酶阳性细胞部分共定位,表明潘氏细胞是产生 Reg3β 的细胞之一。
更新日期:2020-01-10
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