Hydroxytyrosol (HT) and oleuropein (OLE) are natural polyphenols found in extra virgin olive oil. Accumulating evidence indicates that ingestion of olive oil contributes to reduce the risk of cardiovascular diseases and stroke. It has been reported that HT and OLE inhibit human platelet aggregation. We have shown that collagen induces the phosphorylation of heat shock protein 27 (HSP27) in human platelets, resulting in the release of HSP27, an extracellular pro-inflammatory agent. In this study, we investigated the effects of HT and OLE on the collagen-stimulated human platelet activation. The PDGF-AB secretion and the soluble CD40 ligand (sCD40L) release by collagen were reduced by HT or OLE. HT and OLE significantly suppressed the phosphorylation of HSP27 and the release of phosphorylated-HSP27. These findings suggest that olive polyphenol reduces the collagen-stimulated phosphorylation of HSP27 in human platelets and the release. Our results may provide a novel anti- inflammatory effect of olive polyphenol.

中文翻译：

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橄榄多酚可减少胶原蛋白诱导的人血小板中磷酸化HSP27的释放。

羟基酪醇（HT）和橄榄苦苷（OLE）是在特级初榨橄榄油中发现的天然多酚。越来越多的证据表明，摄入橄榄油有助于降低心血管疾病和中风的风险。据报道，HT和OLE抑制人血小板聚集。我们已经表明，胶原蛋白诱导人血小板中热休克蛋白27（HSP27）的磷酸化，导致释放HSP27，一种细胞外促炎剂。在这项研究中，我们调查了HT和OLE对胶原蛋白刺激的人类血小板活化的影响。HT或OLE减少了胶原蛋白的PDGF-AB分泌和可溶性CD40配体（sCD40L）的释放。HT和OLE显着抑制HSP27的磷酸化和磷酸化的HSP27的释放。这些发现表明，橄榄多酚减少了胶原蛋白刺激的人类血小板中HSP27的磷酸化和释放。我们的结果可能提供了橄榄多酚的新型抗炎作用。