Enhanced resistance to oxidative stress makes the adipose stem cells (ASCs) able to promote wound repair and regeneration. Such cells can be achieved by addition of an anti-oxidant to cell culture medium. In this study, a combination of honey (H) and ASCs was applied on burn wounds and the injured area was then covered by a tegaderm (T) dressing in a rat model. Wound healing was evaluated by histopathological, histomorphometrical, molecular, scanning electron microscopy, and biochemical assessments on days 7, 14, and 28 post-wounding. Treatment with ASCs-containing honey (T-H/ASC) resulted in substantial reduction in the level of pro-inflammatory cytokines including interleukin-1β (IL-1β) and transforming growth factor-β1 (TGF-β1) in wound bed, at 7 day post-surgery. T-H/ASCs also elevated the level of bFGF expression, indicating that ASCs enhanced angiogenesis, at 7 day post-wounding. T-H/ASCs significantly improved angiogenesis, re-epithelialization, and granulation tissue formation compared to other treatment regimes, at 14 day post-surgery. These outcomes were in exceptionally good agreement with the histological and biochemical findings. Increased bFGF level in the T-H/ASCs treated wounds at the 28th day post-surgery showed the anti-scarring activity of ASCs. Honey can be considered as a protective material in maintaining the viability of ASCs and improving the cellular resistance to oxidative stress. Furthermore, combination of ASCs and honey can provide a nutrient media for the ASCs and enhance the ability of regeneration of the ASC-based therapies.

中文翻译：

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蜂蜜作为保护材料在烧伤创面愈合中维持脂肪干细胞活力的应用：组织学，分子和生化研究。

增强的抗氧化应激能力使脂肪干细胞（ASC）能够促进伤口修复和再生。可以通过向细胞培养基中添加抗氧化剂来获得此类细胞。在这项研究中，将蜂蜜（H）和ASC的组合应用于烧伤创面，然后在大鼠模型中用Tegaderm（T）敷料覆盖受伤区域。伤口愈合后第7、14和28天通过组织病理学，组织形态计量学，分子，扫描电子显微镜和生化评估评估伤口愈合。在第7天，用含ASCs的蜂蜜（TH / ASC）治疗导致伤口床中促炎细胞因子（包括白介素-1β（IL-1β）和转化生长因子-β1（TGF-β1））的水平大大降低。手术后。TH / ASCs也可提高bFGF表达水平，提示在伤后7天，ASC增强了血管生成。与其他治疗方案相比，在术后14天，TH / ASCs显着改善了血管生成，再上皮形成和肉芽组织形成。这些结果与组织学和生化结果异常吻合。在手术后第28天，经TH / ASCs处理的伤口中bFGF水平升高，显示出ASCs的抗瘢痕形成活性。在维持ASC的生存能力和改善细胞对氧化应激的抵抗力方面，蜂蜜可被视为保护材料。此外，ASC和蜂蜜的结合可以为ASC提供营养介质，并增强基于ASC的疗法的再生能力。与其他治疗方案相比，术后14天的组织和肉芽组织形成情况。这些结果与组织学和生化结果异常吻合。在手术后第28天，经TH / ASCs处理的伤口中bFGF水平升高，显示出ASCs的抗瘢痕形成活性。在维持ASC的生存能力和改善细胞对氧化应激的抵抗力方面，蜂蜜可被视为保护材料。此外，ASC和蜂蜜的结合可以为ASC提供营养介质，并增强基于ASC的疗法的再生能力。与其他治疗方案相比，术后14天的组织和肉芽组织形成情况。这些结果与组织学和生化结果异常吻合。在手术后第28天，经TH / ASCs处理的伤口中bFGF水平升高，显示出ASCs的抗瘢痕形成活性。蜂蜜可被视为维持ASC活力和改善细胞对氧化应激的抵抗力的保护材料。此外，ASC和蜂蜜的结合可以为ASC提供营养介质，并增强基于ASC的疗法的再生能力。在手术后第28天，经TH / ASCs处理的伤口中bFGF水平升高，显示出ASCs的抗瘢痕形成活性。蜂蜜可以被认为是维持ASC活力和改善细胞对氧化应激的抵抗力的保护材料。此外，ASC和蜂蜜的结合可以为ASC提供营养介质，并增强基于ASC的疗法的再生能力。在手术后第28天，经TH / ASCs处理的伤口中bFGF水平升高，显示出ASCs的抗瘢痕形成活性。蜂蜜可被视为维持ASC活力和改善细胞对氧化应激的抵抗力的保护材料。此外，ASC和蜂蜜的结合可以为ASC提供营养介质，并增强基于ASC的疗法的再生能力。