DNA aneuploidy, the altered DNA content of a cell, is a common feature of canine tumors. However, it is unclear whether aneuploid DNA in canine tumor cells show centrosome amplification (CA), which contributes to numerical and structural chromosome aberrations that result in DNA aneuploidy. Here, we evaluated whether DNA aneuploidy and CA occur concurrently in canine tumor cell lines. Centrosome numbers were evaluated in 18 canine tumor cell lines by immunocytochemistry with anti-γ-tubulin antibody, and DNA content was evaluated by flow cytometry using propidium iodide. A total of 15 cell lines showed DNA aneuploidy, and CA was observed in 5 of these 15 cell lines. Together, our results suggest that DNA aneuploidy in canine tumor cells might be explained at least in part by CA. In addition, cell lines with CA may be useful tools to examine the detailed relationship between CA and DNA aneuploidy and the molecular mechanism of CA in canine tumor.

中文翻译：

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犬肿瘤细胞系中的DNA非整倍性和中心体扩增。

DNA非整倍性，即细胞DNA含量的改变，是犬肿瘤的常见特征。但是，尚不清楚犬肿瘤细胞中的非整倍体DNA是否显示中心体扩增（CA），这会导致导致DNA非整倍性的数值和结构染色体畸变。在这里，我们评估了犬肿瘤细胞系中是否同时发生DNA非整倍性和CA。通过抗γ-微管蛋白抗体的免疫细胞化学评估了18种犬肿瘤细胞系的中心体数量，并使用碘化丙啶通过流式细胞术评估了DNA含量。共有15个细胞系显示出DNA非整倍性，在这15个细胞系中的5个中观察到了CA。总之，我们的结果表明，犬肿瘤细胞中的DNA非整倍性可能至少部分由CA解释。此外，