Background: To explore the effects and underlying mechanisms of Morin in human colorectal cancer.Methods: Effects of Morin on proliferation of human colorectal cells in vitro were detected by MTT and colony formation assay. Effects of Morin on growth of colorectal tumors in vivo were examined using the xenograft tumor model. Production of inflammatory cytokines was evaluated by ELISA assay. Effects of Morin on p65-NFκB expression and activation in HCT-116 cells were determined by qRT-PCR and western blot. Effects of C/EBPβ-induced upregulation of p65-NFκB on the inhibitory effects of Morin in HCT-116 cells were evaluated by qRT-PCR, western blot and immunofluorescent staining analysis.Results: Morin inhibited cell proliferation of human colorectal cells in vitro and growth of colorectal tumors in vivo. Morin treatment suppressed TNF-α-induced p65-NFκB expression and activation as well as production of IL-6 and IL-8 in HTC-116 cells. The inhibitory effects of Morin were abrogated by C/EBPβ-induced upregulation of p65-NFκB. Morin had inhibitory effects on human colorectal cancer both in vitro and in vivo.Conclusion: The antitumor functions of Morin in human colorectal cancer cells might be partially due to inhibition of NF-κB signaling.

中文翻译：

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Morin通过抑制NF-κB信号通路来抑制结直肠肿瘤的生长。

背景：探讨桑色素对人大肠癌的作用及其潜在机制。方法：利用MTT和菌落形成法检测桑色素对人大肠癌细胞增殖的影响。使用异种移植肿瘤模型检查了巴戟天对体内大肠肿瘤生长的影响。通过ELISA测定评估炎性细胞因子的产生。用qRT-PCR和western blot检测桑色素对HCT-116细胞中p65-NFκB表达和活化的影响。通过qRT-PCR，western blot和免疫荧光染色分析了C /EBPβ诱导的p65-NFκB上调对Morin在HCT-116细胞中的抑制作用。结果：Morin在体外和体外均抑制人结肠癌细胞的增殖。体内结直肠肿瘤的生长。Morin处理抑制HTC-116细胞中TNF-α诱导的p65-NFκB的表达和激活以及IL-6和IL-8的产生。C /EBPβ诱导的p65-NFκB上调消除了Morin的抑制作用。巴戟天对人大肠癌有抑制作用。结论：巴戟天对人大肠癌细胞的抗肿瘤作用可能部分是由于NF-κB信号通路的抑制。