Perinatal hypoxic-ischemia (HI) is a leading cause of acute mortality and neurologic complications in newborns. Geniposide, a natural product extracted from the herb Gardenia jasminoides, has been shown to possess neuroprotective effects in neurologic deficits. This study aims to investigate whether Geniposide has therapeutic potential to HI brain injury and the underlying mechanisms. C57/bl6 mice were subjected to HI insult on postnatal day 10. Geniposide (20 mg/kg b.w.) was administered intragastrically every day after HI insult for 7 successional days. Then mice at P18 were sacrificed and brain tissues were collected for further analysis. Geniposide treatment significantly inhibited cell apoptosis, reduced serum IgG leakage into brain tissue, attenuated astrogliosis and microgliosis, prevented loss of pericytes, loss of tight junction and adherens junction proteins. The PI3K/Akt signaling pathway, which related proteins were downregulated after HI insult, was activated by Geniposide treatment. Geniposide treatment after neonatal HI insult attenuated HI-induced cell apoptosis, IgG leakage, microgliosis, astrogliosis, pericytes loss and junction protein degradation. Geniposide could protect against HI-induced brain injury, which might be through the activation of PI3K/Akt signaling pathway.

中文翻译：

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栀子苷通过激活 PI3K/Akt 信号通路减轻缺氧缺血后新生小鼠脑损伤

围产期缺氧缺血 (HI) 是新生儿急性死亡和神经系统并发症的主要原因。栀子苷是一种从栀子花中提取的天然产物，已被证明对神经功能障碍具有神经保护作用。本研究旨在探讨栀子苷对 HI 脑损伤是否具有治疗潜力及其潜在机制。C57/bl6 小鼠在出生后第 10 天受到 HI 损伤。在 HI 损伤后每天灌胃给予栀子苷（20 毫克/千克体重），连续 7 天。然后处死 P18 的小鼠并收集脑组织用于进一步分析。栀子苷治疗显着抑制细胞凋亡，减少血清 IgG 渗入脑组织，减轻星形胶质细胞增生症和小胶质细胞增生症，防止周细胞丢失，紧密连接和粘附连接蛋白的丢失。PI3K/Akt 信号通路（其相关蛋白在 HI 损伤后下调）被栀子苷处理激活。新生儿 HI 损伤后的栀子苷治疗减弱了 HI 诱导的细胞凋亡、IgG 渗漏、小胶质细胞增生、星形胶质细胞增生、周细胞丢失和连接蛋白降解。栀子苷可以通过激活 PI3K/Akt 信号通路来预防 HI 诱导的脑损伤。