Minimal plasmids play an essential role in many intermediate steps in molecular biology. For example, they can be used to assemble building blocks in synthetic biology or be used as intermediate cloning plasmids that are ideal for PCR-based mutagenesis methods. A small backbone also opens up for additional unique restriction enzyme cloning sites. Here we describe the generation of pICOz, a 1185-bp fully functional high-copy cloning plasmid with an extended multiple cloning site. We believe that this is the smallest high-copy cloning vector ever described.

中文翻译：

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从具有扩展的多个克隆位点的pUC18质粒主链工程化最小克隆载体。

最小质粒在分子生物学的许多中间步骤中起着至关重要的作用。例如，它们可以用于在合成生物学中组装构件，或用作中间克隆质粒，是基于PCR的诱变方法的理想选择。小骨架也为其他独特的限制酶克隆位点打开了大门。在这里，我们描述了pICOz的产生，pICOz是具有扩展的多个克隆位点的1185 bp全功能高拷贝克隆质粒。我们认为这是有史以来最小的高拷贝克隆载体。