A major challenge for microbiome studies is maintaining an even and accurate DNA extraction in the presence of samples with a wide range of bacterial content. Here we compare five DNA extraction methods using replicate stool samples that were diluted to create high and low biomass samples. Our results indicate greater variation in microbiome composition between high and low biomass samples than variation between methods. Many of the extraction methods had reduced yield from low biomass samples; however, our adapted plate column-based extraction method was evenly efficient and captured the largest number of high-quality reads. Based on these results, we have identified a DNA extraction method that ensures adequate yield in metagenomic microbiome studies that have samples with a broad range of bacterial content.

中文翻译：

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随着微生物生物量的变化，提高了微生物组研究中DNA提取的产量和准确性。

微生物组研究的主要挑战是在存在多种细菌含量的样品的情况下，如何保持均匀，准确的DNA提取。在这里，我们比较了使用复制的粪便样品（稀释后可产生高和低生物量样品）的五种DNA提取方法。我们的结果表明，高和低生物量样品之间微生物组组成的差异要大于方法之间的差异。许多提取方法降低了低生物量样品的产量。但是，我们改编的基于板式色谱柱的提取方法效率很高，并且捕获了大量的高质量读物。根据这些结果，我们确定了一种DNA提取方法，该方法可确保宏基因组微生物组研究中的细菌含量广泛的样本具有足够的产量。