After surgical resection for a bone tumor, the uncleared bone tumor cells can multiply and cause recurrence of the bone tumor. It is worthwhile to design a scaffold that kills the remaining bone tumor cells and repairs bone defects that were given rise to by surgical resection. Additionally, it is extremely important to consider the function of angiogenesis in the process of bone regeneration because the newly formed blood vessels can offer the nutrients for bone regeneration. In this work, a novel metal-organic framework Cu-TCPP nanosheets interface-structured β-tricalcium phosphate (TCP) (Cu-TCPP-TCP) scaffold was successfully prepared through integrating a 3D-printing technique with an in-situ growth method in a solvothermal system. Owing to the excellent photothermal effect of Cu-TCPP nanosheets, Cu-TCPP-TCP scaffolds that were illuminated by near-infrared (NIR) light demonstrated photothermal performance, which was well regulated through varying the contents of Cu-TCPP nanosheets, and the ambient humidity and power density of NIR light. When cultured with osteosarcoma cells, Cu-TCPP-TCP scaffolds killed a significant quantity of osteosarcoma cells through released heat energy after exposure to NIR light with power density 1.0 W cm-2 and duration 10 min. Similarly, Cu-TCPP-TCP scaffolds ablated subcutaneous bone tumor tissues on the backs of naked mice and suppressed their growth because of the heat energy transformed from NIR light. I n-vitro studies found that Cu-TCPP-TCP scaffolds ably supported the attachments of both human bone marrow stromal cells (HBMSCs) and human umbilical vein endothelial cells (HUVECs), and significantly stimulated expressions of osteogenesis differentiation-related genes in HBMSCs and angiogenesis differentiation-related genes in HUVECs. After implanting Cu-TCPP-TCP scaffolds into the bone defects of rabbits, they effectively promoted bone regeneration. Thus, the integration of the bone-forming bioactivity of TCP scaffolds with the photothermal properties of Cu-TCPP nanosheets and angiogenesis activity of Cu ions makes Cu-TCPP-TCP scaffolds multifunctional, representing a new horizon to develop biomaterials for simultaneously curing bone tumors and repairing bone defects.

中文翻译：

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金属-有机框架纳米片结构支架的3D打印以及肿瘤治疗和骨骼构造。

在对骨肿瘤进行外科手术切除后，未清除的骨肿瘤细胞会繁殖并导致骨肿瘤复发。值得设计的支架可以杀死剩余的骨肿瘤细胞并修复因手术切除而引起的骨缺损。另外，在新生血管中考虑血管生成的功能极为重要，因为新形成的血管可以为新生骨骼提供营养。在这项工作中，通过将3D打印技术与原位生长方法相结合，成功地制备了一种新型的金属有机框架Cu-TCPP纳米片界面结构化的β-磷酸三钙（TCP）（Cu-TCPP-TCP）支架。溶剂热系统。由于Cu-TCPP纳米片具有出色的光热效应，用近红外（NIR）照明的Cu-TCPP-TCP支架表现出光热性能，通过改变Cu-TCPP纳米片的含量以及环境湿度和NIR光的功率密度可以很好地调节光热性能。当与骨肉瘤细胞一起培养时，Cu-TCPP-TCP支架暴露于功率密度为1.0 W cm-2且持续时间为10分钟的NIR光后，通过释放的热能杀死了大量的骨肉瘤细胞。同样，Cu-TCPP-TCP支架可消融裸鼠背部的皮下骨肿瘤组织，并由于NIR光转换出的热能而抑制了其生长。体外研究发现，Cu-TCPP-TCP支架可很好地支持人骨髓基质细胞（HBMSC）和人脐静脉内皮细胞（HUVEC）的附着，并显着刺激了HBMSCs中成骨分化相关基因和HUVECs中血管生成分化相关基因的表达。将Cu-TCPP-TCP支架植入兔的骨缺损后，它们有效地促进了骨再生。因此，TCP支架的骨形成生物活性与Cu-TCPP纳米片的光热特性和Cu离子的血管生成活性的整合使Cu-TCPP-TCP支架具有多功能性，代表了开发同时治愈骨肿瘤和骨骼的生物材料的新视野。修复骨缺损。