Abstract Microbial contamination during fuel storage can cause fuel system fouling and corrosion. Characterizing microbial contamination is critical for preventing and solving these problems. In this study, culture-based combing with the culture-independent methods, were used to profile the microbial contamination in aviation fuel. High-throughput sequencing (HTS) modified by propidium monoazide (PMA) revealed a higher diversity of contaminating microorganisms in samples than the culture method. Proteobacteria (47%), Actinobacteria (21%) and Ascomycota (>99%, fungi) were the most abundant phyla, and the neglected archaea was also detected. Additionally, qPCR-based methods revealed all samples contained a heavy level of microbial contamination, which was more accurate than its culturable counterparts, and fungal contamination was still a problem in aviation fuel. The application of a PCR-based method gives deeper insight into microbial contamination in aviation fuel than the conventional culture method, thus using it for regular detection and accurate description of fuel contamination is strongly recommended in the case of explosive microbial growth.

中文翻译：

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分析机场航空​​燃料中的微生物污染

摘要 燃料储存过程中的微生物污染会导致燃料系统结垢和腐蚀。表征微生物污染对于预防和解决这些问题至关重要。在这项研究中，基于培养的方法与不依赖培养的方法相结合，用于分析航空燃料中的微生物污染。由单叠氮化丙啶 (PMA) 修饰的高通量测序 (HTS) 显示，与培养方法相比，样品中污染微生物的多样性更高。Proteobacteria (47%)、Actinobacteria (21%) 和 Ascomycota (>99%，真菌) 是最丰富的门，也检测到被忽视的古菌。此外，基于 qPCR 的方法显示所有样品都含有大量微生物污染，这比其可培养的对应物更准确，真菌污染仍然是航空燃料中的一个问题。与传统培养方法相比，基于 PCR 的方法可以更深入地了解航空燃料中的微生物污染，因此在微生物爆炸性增长的情况下，强烈建议将其用于定期检测和准确描述燃料污染。