Seleno-short-chain chitosan (SSCC) is a derivative of chitosan. In the present study, we sought to investigate the underlying antitumor mechanism of SSCC on human gastric cancer BGC-823 cells in vitro. MTT assay suggested that SSCC exhibited a dose-dependent inhibitory effect on the proliferation of BGC-823 cells. We found the SSCC-treated cells showed typical morphological characteristics of apoptosis in a dose dependent manner by observing on microscope. Annexin V-FITC/PI double staining and cell cycle assay identified that SSCC could induce BGC-823 cells apoptosis by triggering G2/M phase arrest. Our research provided the first evidence that SSCC could effectively induce the apoptosis of BGC-823 cells via an intrinsic mitochondrial pathway, as indicated by inducing the disruption of mitochondrial membrane potential (MMP), the excessive accumulation of reactive oxidative species (ROS), the increase of Bax/Bcl-2 ratio and the activation of caspase 3, caspase 9 and cytochrome C (Cyt-C) in BGC-823 cells. These combined results clearly indicated that SSCC could induce BGC-823 cells apoptosis by the involvement of mitochondrial signaling pathway, which provided precise experimental evidence for SSCC as a potential agent in the prevention and treatment of human gastric cancer.

中文翻译：

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硒短链壳聚糖（SSCC）对人胃癌BGC-823细胞的抗肿瘤作用。

硒短链壳聚糖（SSCC）是壳聚糖的衍生物。在本研究中，我们试图研究SSCC对人胃癌BGC-823细胞的潜在抗肿瘤机制。MTT测定表明SSCC对BGC-823细胞的增殖具有剂量依赖性的抑制作用。我们通过显微镜观察发现，经SSCC处理的细胞以剂量依赖性方式显示出典型的细胞凋亡形态学特征。Annexin V-FITC / PI双重染色和细胞周期分析表明，SSCC可以通过触发G2 / M期阻滞来诱导BGC-823细胞凋亡。我们的研究提供了第一个证据，表明SSCC可以通过内在的线粒体途径有效诱导BGC-823细胞凋亡，这可以通过诱导线粒体膜电位（MMP）破坏来表明，BGC-823细胞中活性氧化物质（ROS）的过度积累，Bax / Bcl-2比的增加以及caspase 3，caspase 9和细胞色素C（Cyt-C）的激活。这些综合结果清楚地表明，SSCC可通过线粒体信号传导途径参与诱导BGC-823细胞凋亡，这为SSCC作为预防和治疗人胃癌的潜在药物提供了精确的实验证据。