Abstract Psychrotrophic Pseudomonas species form biofilms on meat during refrigerated and temperature abuse conditions. Biofilm growth leads to slime formation on meat which is a key organoleptic degradation characteristic. Limited research has been undertaken characterising biofilms grown on meat during chilled aerobic storage. In this work, biofilms formed by two key meat spoilage organisms, Pseudomonas fragi and Pseudomonas lundensis were studied in situ using five strains from each species. Biofilm structures were studied using confocal microscope images, cellular arrangement, cell counts and biomass quantifications. This work demonstrated that highly dense, compact biofilms are a characteristic of P. fragi strains. P. lundensis formed biofilms with loosely arranged cells. The cells in P. fragi biofilm appear to be vertically oriented whereas this characteristic was absent in P. lundensis biofilms formed under identical conditions. Despite the continued access to nutrients, biofilms formed on meat by proteolytic Pseudomonas species dispersed after a population maximum was reached.

中文翻译：

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低温肉类腐败假单胞菌形成的生物膜的原位表征

摘要 嗜冷假单胞菌属物种在冷藏和温度滥用条件下在肉上形成生物膜。生物膜生长导致肉上形成粘液，这是关键的感官降解特征。已经进行了有限的研究来表征冷冻有氧储存过程中肉上生长的生物膜。在这项工作中，使用来自每个物种的五个菌株原位研究了由两种主要肉类腐败生物形成的生物膜，脆弱假单胞菌和伦德假单胞菌。使用共聚焦显微镜图像、细胞排列、细胞计数和生物量量化研究生物膜结构。这项工作表明高密度、致密的生物膜是 P. fragi 菌株的特征。P. lundensis 形成具有松散排列细胞的生物膜。P 中的细胞。fragi 生物膜似乎是垂直取向的，而在相同条件下形成的 P. lundensis 生物膜中不存在这种特征。尽管继续获得营养，但在达到种群最大值后，蛋白水解假单胞菌物种在肉上形成的生物膜分散。