The herb Ruta chalepensis L. exhibits medical effects, such as anti-inflammatory, central nervous system depressant, and antipyretic activities. However, a genetic transformation method has not yet been developed for this species. In this paper, a simple and efficient tissue culture and genetic transformation system for R. chalepensis is reported. An amyloid β-peptide (Aβ) gene, which is considered to be a causative agent of Alzheimer's disease (AD), fused with green-fluorescent protein (GFP), was introduced into R. chalepensis. When the leaves of R. chalepensis expressing Aβ-GFP were administered orally to C57BL/6J mice, serum anti-Aβ antibody titers of several mice were elevated without the use of an adjuvant. These results indicated that an oral vaccine against AD using R. chalepensis may be feasible. R. chalepensis is rich in bioactive compounds that may have synergistic effects with the vaccine for AD. Plant-derived vaccines are safer and cheaper than those produced from animal cells or microbes, because plants can serve as biofactories at low cost and with high biosynthetic capacity.

中文翻译：

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表达淀粉样蛋白 β-GFP 融合蛋白的草本植物 Ruta chalepensis L. 的生产。

芸香草具有抗炎、中枢神经系统抑制和解热等功效。然而，尚未开发出针对该物种的遗传转化方法。本文报道了一种简单、高效的查氏罗勒氏菌组织培养和遗传转化系统。β-淀粉样蛋白肽（Aβ）基因被认为是阿尔茨海默氏病（AD）的致病因子，与绿色荧光蛋白（GFP）融合，被引入查氏雷氏菌中。当对C57BL/6J小鼠口服表达Aβ-GFP的R. chalepensis叶子时，在不使用佐剂的情况下，几只小鼠的血清抗Aβ抗体滴度升高。这些结果表明，使用查勒彭氏菌口服 AD 疫苗可能是可行的。R. chalepensis 富含生物活性化合物，可能与 AD 疫苗产生协同作用。植物源疫苗比动物细胞或微生物生产的疫苗更安全、更便宜，因为植物可以作为生物工厂，成本低廉，生物合成能力高。