When ingested as a dietary supplement, Ganoderma lucidum spore powders (GLSP) provide various health benefits such as enhanced immunity, liver protection and anti-cancer effects. In this study, triterpenoid extraction from GLSP was achieved using an ultrasound-assisted process which was optimized using response surface methodology (RSM). Ultrasound-assisted extraction (UAE) was also compared to the most conventional chemical extraction method. For UAE, optimum extraction conditions were found to be ethanol concentration = 95% v/v; solvent to solid ratio = 50:1 mL/g; ultrasound time = 5.4 min; ultrasound power = 564.7 w, and ultrasound probe distance = 8.2 cm. At optimal UAE conditions, no significant differences were found between experimental (0.97 ± 0.04 %) and predicted values (99%); which indicates appreciable correlation at the 97% confidence interval. The findings show the application of Box-Behnken design (BBD) to predict and optimize triterpenoid yield for UAE of triterpenoid from GLSP. Furthermore, glucose consumption was 2.68 times that of control samples when tested with insulin-resistant HepG2 cell, showing potential use in type 2 diabetes. In addition, triterpenoid extracts show good biocompatibility and inhibition of antioxidant activity.

中文翻译：

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使用超声波辅助提取从灵芝孢子粉生产三萜类化合物。

当作为膳食补充剂摄入时，灵芝孢子粉（GLSP）具有多种健康益处，例如增强的免疫力，肝脏保护作用和抗癌作用。在这项研究中，使用超声辅助过程从GLSP中提取三萜类化合物，该过程使用响应表面方法（RSM）进行了优化。超声辅助萃取（UAE）也与最常规的化学萃取方法进行了比较。对于阿联酋，发现最佳提取条件为乙醇浓度= 95％v / v；溶剂与固体的比例= 50：1 mL / g；超声时间= 5.4分钟；超声功率= 564.7 w，超声探头距离= 8.2 cm。在最佳阿联酋条件下，实验值（0.97±0.04％）与预测值（99％）之间无显着差异；这表明置信区间为97％时具有明显的相关性。研究结果表明Box-Behnken设计（BBD）在预测和优化GLSP中三萜类化合物的阿联酋三萜产量方面的应用。此外，当用胰岛素抵抗性HepG2细胞测试时，葡萄糖消耗量是对照样品的2.68倍，表明其在2型糖尿病中的潜在用途。此外，三萜类提取物显示出良好的生物相容性并抑制抗氧化活性。