Canine brucellosis is an infectious and contagious disease associated with reproductive losses in breeding kennels. As a zoonotic disease, it poses a risk to human health, especially for veterinarians and breeders who handle materials potentially contaminated with Brucella canis. However, canine brucellosis is a neglected and underestimated disease given the difficulties in establishing a definitive diagnosis. We evaluated the frequency of detection of B. canis in 5 breeding kennels by using various serologic methods and PCR. Circulation of B. canis in these kennels was confirmed by bacterial isolation. The frequency of positive serologic results varied from 6.3% by AGID to 16.5% by dot-ELISA. There was no positive serology for smooth Brucella. PCR testing was positive in 13.9% of samples. The only detection tests with reasonable agreement were PCR and 2ME-MAT. The diagnosis of canine brucellosis remains challenging. The use of a single laboratory method, or even the use of different laboratory methods, may not be sufficient to reach a definitive diagnosis.

中文翻译：

---

犬布鲁氏菌病的诊断：各种血清学检测和 PCR 的比较。


犬布鲁氏菌病是一种与繁殖犬舍繁殖损失相关的传染病。作为一种人畜共患疾病，它对人类健康构成风险，特别是对于处理可能被犬布鲁氏菌污染的材料的兽医和饲养员而言。然而，由于难以明确诊断，犬布鲁氏菌病是一种被忽视和低估的疾病。我们使用各种血清学方法和 PCR 评估了 5 个饲养场中犬双歧杆菌的检测频率。通过细菌分离证实了这些犬舍中存在犬芽胞杆菌的循环。阳性血清学结果的频率从 AGID 的 6.3% 到 dot-ELISA 的 16.5% 不等。平滑布鲁氏菌没有血清学阳性。 13.9% 的样本 PCR 检测呈阳性。唯一具有合理一致性的检测测试是 PCR 和 2ME-MAT。犬布鲁氏菌病的诊断仍然具有挑战性。使用单一实验室方法，甚至使用不同的实验室方法，可能不足以得出明确的诊断。