Lycopene is a highly valued carotenoid with wide applications in various industries. The market demand for lycopene promotes research in metabolic engineering of heterologous hosts for lycopene. In this study, Pichia pastoris strain GS115 was genetically engineered to produce lycopene by integrating the heterologous lycopene biosynthesis genes from Corynebacterium glutamicum ATCC13032. The resulting strain, L1, produced 0.115 mg/g cell dry weight (DCW) lycopene. Through optimization by promoter selection, improving the precursor supply and expanding the Geranylgeranyl diphosphate (GGPP) pool, ultimately, the lycopene yield of the final optimal strain was 6.146 mg/g DCW with shake flask fermentation and 9.319 mg/g DCW (0.714 g/L) in a 3 L fermenter. The lycopene yield in this study is the highest yield of lycopene in P. pastoris reported to date, which demonstrated the potential of P. pastoris in lycopene synthesis and as a candidate host organism for the synthesis of other high value-added terpenoids.

中文翻译：

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通过代谢工程毕赤酵母生产番茄红素。

番茄红素是一种非常有价值的类胡萝卜素，在各个行业都有广泛的应用。番茄红素的市场需求促进了番茄红素异源宿主代谢工程的研究。在这项研究中，通过整合来自谷氨酸棒杆菌ATCC13032的异源番茄红素生物合成基因，对巴斯德毕赤酵母菌株GS115进行了基因工程改造以生产番茄红素。所得菌株L1产生0.115 mg / g细胞干重（DCW）番茄红素。通过选择启动子进行优化，改善前体供应并扩大Geranylgeranyl diphosphate（GGPP）库，最终，最终最佳菌株的番茄红素产量为6.146 mg / g DCW（摇瓶发酵）和9.319 mg / g DCW（0.714 g / L）放在3 L的发酵罐中。这项研究中的番茄红素产量是迄今为止报道的巴斯德毕赤酵母中番茄红素的最高产量，