Tetraselmis is a green algal genus, some of whose species are important in aquaculture as well as biotechnology. In algal culture, fluorescent lamps, traditional light source for culturing algae, are now being replaced by a cost-effective light-emitting diodes (LEDs). In this study, we investigated the effect of LED light of different wavelengths (white, red, yellow, and blue) on the growth of Tetraselmis suecica and its associated microbial community structures using the next-generation sequencing (NGS). The fastest growth rate of T. suecica was shown in the red light, whereas the slowest was in yellow. The highest OTUs (3426) were identified on day 0, whereas the lowest ones (308) were found on day 15 under red light. The top 100 OTUs associated with day 0 and day 5 cultures of T. suecica under the red and yellow LED were compared. Only 26 OTUs were commonly identified among four samples. The highest numbers of unique OTUs were identified at day 0, indicating the high degree of initial microbial diversity of the T. suecica inoculum. The red light-unique OTUs occupied 34.98%, whereas the yellow-specific OTUs accounted for only 2.2%. This result suggested a higher degree of interaction in T. suecica culture under the red light, where stronger photosynthesis occurs. Apparently, the microbial community associated with T. suecica related to the oxygen produced by algal photosynthesis. This result may expand our knowledge about the algaebacteria consortia, which would be useful for various biotechnological applications including wastewater treatment, bioremediation, and sustainable aquaculture.

中文翻译：

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使用下一代测序技术，评估不同LED灯下与Tetraselmis suecica培养相关的微生物群落的动力学。

Tetraselmis是绿色藻类，其中一些物种在水产养殖和生物技术中都很重要。在藻类养殖中，荧光灯（用于培养藻类的传统光源）现在已被具有成本效益的发光二极管（LED）取代。在这项研究中，我们使用下一代测序（NGS）研究了不同波长（白色，红色，黄色和蓝色）的LED灯对Tetraselmis suecica及其相关微生物群落结构生长的影响。最快的T. suecica增长率以红灯显示，而最慢的以黄色显示。在第0天发现最高的OTU（3426），而在第15天在红灯下发现最低的OTU（308）。比较了红色和黄色LED下与T. suecica的第0天和第5天培养物相关的前100个OTU。在四个样本中，通常仅识别出26个OTU。在第0天鉴定出最高数量的独特OTU，这表明suecica接种物的初始微生物多样性很高。独特的红色OTU占34.98％，而特定于黄色的OTU仅占2.2％。该结果表明，在红色光下，在光合作用较强的条件下，苏梅克锥虫培养物中的相互作用程度更高。显然，与T. suecica与藻类光合作用产生的氧气有关。该结果可能会扩展我们对藻类细菌财团的了解，这对于包括废水处理，生物修复和可持续水产养殖在内的各种生物技术应用将是有用的。