In order to explore the salt-stress responses of two rice varieties, the physiological responses and biochemical responses were investigated using proteomics and classical biochemical methods. The results showed that the seedling growth was inhibited under salt condition in two rice varieties, the seedling growth in the tolerant variety was better than the sensitive variety. The sensitive variety(L7) appeared obvious salt-injury under 3-day salt stress, the tolerant variety (T07339) keep normal growth under 7-day salt stress except that the shoot length was decreased. Through the growth-parameters analysis, most of them in L7 were restrained by salinity and most in T07339 were unaffected. In T07339, the fresh root weight, the content of chlorophyll and the fresh shoot weight were even increased after 7 days of salt stress. A comparison of two-dimensional gel electrophoresis (2-DGE) protein profiles revealed 8 differently expressed proteins. Four proteins were expressed in different pattern between sensitive and tolerant varieties. These results provide novel insights into the investigations of the salt-response proteins that involved in improved salt tolerance.

中文翻译：

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两个水稻品种苗期盐响应分析。

为了探究两个水稻品种的盐胁迫反应，利用蛋白质组学和经典生化方法研究了其生理反应和生化反应。结果表明，盐条件下两个水稻品种的幼苗生长均受到抑制，耐盐品种的幼苗生长优于敏感品种。敏感品种(L7)在盐胁迫3 d后出现明显的盐害，耐性品种(T07339)在盐胁迫7 d后仍保持正常生长，但芽长有所缩短。通过生长参数分析，L7中的大部分受到盐度的抑制，而T07339中的大部分则不受影响。T07339在盐胁迫7 d后，根鲜重、叶绿素含量和鲜芽重均有所增加。二维凝胶电泳 (2-DGE) 蛋白质图谱的比较揭示了 8 种不同表达的蛋白质。四种蛋白质在敏感品种和耐受品种之间以不同的模式表达。这些结果为涉及改善耐盐性的盐响应蛋白的研究提供了新的见解。