Hepatitis E virus (HEV) can lead to high mortality during pregnancy. This study was to investigate the adverse pregnancy outcomes caused by different HEV genotypes and their prevention by HEV 239 vaccine in rabbits. Forty-two female rabbits were randomly and equally divided into 7 groups (A-G). HEV 239 vaccine and a placebo were administered to groups E (10 μg×2), F (5 μg×2) and G (1 mL of PBS×2) before copulation. After pregnancy, 1 mL of 1.5×106 copies/mL rabbit HEV3 was inoculated to groups A, E, F and G, swine HEV4/human HEV3 to groups B/C, and group D was a negative control. Anti-HEV antibody, HEV RNA, and alanine aminotransferase (ALT)/aspartate aminotransferase (AST) levels were monitored. Pregnant rabbits infected by HEV manifested HEV infection symptoms including fecal virus shedding, ALT/AST elevation, and histopathological changes, and adverse pregnancy outcomes. Immunized pregnant rabbits in groups E and F showed no HEV infection symptoms and adverse outcomes. The newborn rabbits delivered by pregnant rabbits with/without immunization showed without/with HEV infection symptoms. This study demonstrated that multiple genotypes of HEV infection can cause adverse outcomes and HEV 239 vaccine can prevent HEV-related adverse outcomes in pregnant rabbits.

中文翻译：

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与戊型肝炎相关的不良妊娠结局及其在兔模型中的戊型肝炎疫苗预防。

戊型肝炎病毒（HEV）可能导致怀孕期间的高死亡率。本研究旨在探讨不同基因型HEV引起的不良妊娠结局以及HEV 239疫苗对兔的预防作用。将42只雌性兔子随机且均等地分为7组（AG）。在交配之前，将HEV 239疫苗和安慰剂分别施用于E组（10μg×2），F组（5μg×2）和G组（1 mL PBS×2）。怀孕后，将A，E，F和G组分别接种1 mL 1.5×106拷贝/ mL兔HEV3，将B组/ C组接种猪HEV4 /人HEV3，D组作为阴性对照。监测抗HEV抗体，HEV RNA和丙氨酸氨基转移酶（ALT）/天冬氨酸氨基转移酶（AST）的水平。受戊型肝炎病毒感染的怀孕兔子表现出戊型肝炎病毒感染症状，包括粪便病毒脱落，ALT / AST升高以及组织病理学改变，和不良的妊娠结局。E和F组中的免疫怀孕兔未显示HEV感染症状和不良后果。妊娠兔经免疫接种或未免疫接种后所生的新生兔均表现出无/有HEV感染症状。这项研究表明，多种基因型的戊型肝炎病毒感染可导致不良后果，而戊型肝炎疫苗239疫苗可预防妊娠兔的戊型肝炎相关不良后果。