Background

Sertraline (SRT) is an antidepressant that has proven its activity in vitro against Cryptococcus, Coccidioides, Trichosporon and other fungi. Disseminated sporotrichosis, although rare, has a high mortality and its treatment is difficult and prolonged, often relying in combining two or more antifungals.

Aims

In our study we evaluate the antifungal activity of SRT, alone and in combination with itraconazole (ITC), voriconazole (VRC) and amphotericin B (AMB), against 15 clinical isolates of Sporothrix schenckii.

Methods

We used the broth microdilution method as described by the CLSI to test the susceptibility to antifungals, and the checkerboard microdilution method to evaluate drug interactions.

Results

The minimum inhibitory concentration (MIC) with SRT was in the range of 4–8 μg/ml, while for AMB, VRC and ITC were 0.5–4 μg/ml, 0.5–8 μg/ml and 0.125–2 μg/ml, respectively. In addition, SRT showed synergy with ITC in one strain, mainly additivity with VRC, and indifference with AMB in others.

Conclusions

The MIC values with SRT for the isolates studied show the potential role of this drug as an adjuvant in the treatment of sporotrichosis, especially in disseminated or complicated cases.

中文翻译：

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舍曲林对Sporothrix schenckii临床分离株的体外抑制活性。

背景

舍曲林（SRT）是已被证明在体外其活性针对抗抑郁隐球菌，球孢子菌属，毛孢子和其它真菌。弥散性孢子丝菌病虽然很少见，但死亡率高，治疗困难且时间长，常常依靠结合两种或多种抗真菌药。

目的

在我们的研究中，我们评估了SRT单独或与伊曲康唑（ITC），伏立康唑（VRC）和两性霉素B（AMB）结合使用对15株申氏孢子菌的抗真菌活性。

方法

我们使用了CLSI所述的肉汤微稀释法来测试对真菌的敏感性，而棋盘微稀释法则用来评估药物的相互作用。

结果

SRT的最低抑菌浓度（MIC）为4–8μg/ ml，而AMB，VRC和ITC的最低抑菌浓度为0.5–4μg/ ml，0.5–8μg/ ml和0.125–2μg/ ml，分别。此外，SRT在一种菌株中表现出与ITC的协同作用，主要表现出与VRC的可加性，而另一种表现出对AMB的漠不关心。

结论

研究的分离物的SRT MIC值表明该药作为佐剂在治疗孢子虫病中的潜在作用，尤其是在散播或复杂病例中。