Non-homologous end joining (NHEJ), a process for repair of DNA-breaks that does not require a DNA-template, involves synapsis, end-processing and ligation. Synapsis is initiated by assembly of the Ku-heterodimer on DNA broken ends, followed by the formation of DNA-dependent protein kinase (DNA-PK) - an assembly of the catalytic subunit (DNA-PKcs), the Ku-heterodimer and DNA. Recent progress in understanding the structural architecture of DNA-PK, achieved by X-ray crystallography and cryo-electron microscopy, has revealed a stage of DNA-PKcs on which other components from the pathway assemble and mediate kinase activity allosterically. This review provides a comparative overview of recently published structures of DNA-PK, together with a discussion of other complexes mediated by the Ku heterodimer. It also shows that some binders are specific to particular patho-physiological conditions.

中文翻译：

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NHEJ在DNA双链断裂修复中的多组分组装。

非同源末端连接（NHEJ）是一种不需要DNA模板的DNA断裂修复过程，涉及突触，末端加工和连接。突触通过在DNA断裂末端装配Ku-异二聚体开始，然后形成DNA依赖性蛋白激酶（DNA-PK）-催化亚基（DNA-PKcs），Ku-异二聚体和DNA的装配。通过X射线晶体学和低温电子显微镜在了解DNA-PK的结构结构方面的最新进展，揭示了DNA-PKcs的一个阶段，在该阶段，来自该途径的其他成分组装并介导变构激酶活性。这篇综述提供了最近发表的DNA-PK结构的比较概述，以及对Ku异二聚体介导的其他复合物的讨论。